The effect of local subcutaneous delivery of vascular endothelial growth factor on the function of a chronically implanted amperometric glucose sensor.
The effect of local subcutaneous delivery of vascular endothelial growth factor on the function of a chronically implanted amperometric glucose sensor.
复制标题
局部皮下输送血管内皮生长因子对长期植入的电流型葡萄糖传感器功能的影响。
DOI：
10.1089/152091504773731320
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Federiuk,IsaacF
中科院分区：
文献类型：
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作者：
Ward,WKenneth;Wood,MichaelD;Casey,HeatherM;Quinn,MatthewJ;Federiuk,IsaacF
在植入器械（如葡萄糖传感器）周围形成的异物囊是血管减少的，对葡萄糖的渗透性有限。如果血管化良好，这样的囊可以更好地发挥功能。我们 假设通过局部释放血管内皮生长因子（VEGF）实现的囊状血管形成将导致功能增强。安培葡萄糖传感器阵列磁盘，每个磁盘有四个 指示电极植入大鼠体内。动物通过渗透泵在传感器表面上距离其中一个电极2mm的位置处接受局部皮下输注VEGF 165（“近 单位”）。“中间”电极单位为15 mm，“远端”单位为22 mm，距离VEGF源。每2周输注一次葡萄糖，通过遥测评估探头功能。附近的单位展示 较低的滞后时间（血糖后延迟）比中间和遥远的单位。近距离单位的平均绝对相对差异小于远距离单位。中的数据对的百分比 近感测单元的Clarke误差网格A区大于远感测单元的Clarke误差网格A区。生理盐水对照的功能测量值介于近和远VEGF值之间。 在紧邻VEGF输注端口的单元中，发现葡萄糖探头功能更有利。这一发现最可能的原因是周围异物中新血管生长增加。 身体胶囊血管生成生长因子的缓慢释放可能是一种潜在的方法，用于长期增强皮下植入的生物传感器的功能。
The foreign body capsule that forms around implanted devices such as glucose sensors is hypovascular and has limited permeability to glucose. Such a capsule may function better if well vascularized. We hypothesized that capsular vascularization achieved by local release of vascular endothelial growth factor (VEGF) would lead to enhanced function. Amperometric glucose sensor array disks, each with four indicating electrodes, were implanted into rats. Animals received local subcutaneous infusions of VEGF165via osmotic pumps at a location on the sensor face 2 mm from one of the electrodes ("near units"). "Intermediate" electrode units were 15 mm, and "distant" units were 22 mm, from the VEGF source. Every 2 weeks, a glucose infusion was given to assess sensor function by telemetry. Near units demonstrated a lower lag duration (delay after blood glucose) than intermediate and distant units. The mean absolute relative difference for near units was less than for distant units. The percentage of data pairs in the A region of the Clarke error grid of the near sensing units was greater than that of the distant units. Values for the functional measures for saline controls fell between near and distant VEGF values. Glucose sensor function was found to be more favorable in units immediately adjacent to the VEGF infusion port. The most likely cause for this finding is increased neovessel growth in the surrounding foreign body capsule. Slow release of angiogenic growth factors may be a potential method for chronically enhancing the function of a subcutaneously implanted biosensor.