Surfactant-free biodegradable polymeric nanoparticles generated from self-organized precipitation route: Cellular uptake and cytotoxicity
Surfactant-free biodegradable polymeric nanoparticles generated from self-organized precipitation route: Cellular uptake and cytotoxicity
复制标题
自组织沉淀途径产生的不含表面活性剂的可生物降解聚合物纳米颗粒：细胞摄取和细胞毒性
DOI：
10.1016/j.eurpolymj.2014.05.017
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发表时间：
2014-08-01
影响因子：
6
通讯作者：
Tao, Juan
中科院分区：
文献类型：
--
作者：
Liang, Ruijing;Dong, Liyun;Tao, Juan
采用自组织沉淀（SORP）法制备了尺寸均匀、不含表面活性剂的可生物降解聚合物纳米颗粒（NPs）。通过改变制备条件可以轻松调整纳米颗粒的尺寸和尺寸分布。更重要的是，我们证明疏水性物质、半导体纳米晶体和磁性纳米粒子可以有效、定量地封装到聚合物纳米粒子中，以生成多功能杂化纳米粒子。制备过程中不使用表面活性剂，这对于形成的聚合物纳米颗粒用于生物相关应用至关重要。为了评估表面活性剂对细胞行为的影响，进行了不含表面活性剂的纳米粒子和表面活性剂包被的纳米粒子的细胞摄取和细胞毒性。我们的研究结果表明，与乳液-溶剂蒸发法制备的残留表面活性剂包被的纳米颗粒相比，不含表面活性剂的纳米颗粒在体外能够更迅速、更有效地被细胞吞噬，证明不含表面活性剂的纳米颗粒在细胞摄取方面更具优势，在药物递送和生物成像方面更安全。此外，表面活性剂包被的纳米粒子抑制了纳米粒子的细胞摄取，并且对黑色素瘤A875细胞而不是人脐静脉内皮细胞（EVC-304）具有选择性毒性，特别是由于细胞内活性氧（ROS）的产生而对表面活性剂聚氧乙烯辛基苯基醚（Triton X-100）产生选择性毒性。由 SORP 制备的不含表面活性剂的均匀纳米粒子，结合了疏水性药物和功能材料的理想特性，可能在同时药物输送、成像和磁场操纵应用中具有优势。
Surfactant-free biodegradable polymeric nanoparticles (NPs) with uniform sizes were prepared by self-organized precipitation (SORP) method. Size and size distribution of the NPs can be easily tuned by varying the preparation conditions. More importantly, we demonstrate that hydrophobic species, semiconductor nanocrystals, and magnetic NPs can be encapsulated into the polymeric NPs effectively and quantitatively to generate multifunctional hybrid NPs. No surfactant is employed during the preparation process, which is crucial for the formed polymeric NPs to be used in bio-related applications. To evaluate the influence of surfactants on cellular behavior, cellular uptake and cytotoxicity of surfactant-free NPs and surfactant-coated NPs were performed. Our results indicated that surfactant-free NPs could be more promptly and effectively phagocytized by cells in vitro compared to residual surfactant-coated NPs prepared from the emulsion-solvent evaporation method, providing a proof that the surfactant-free NPs have more advantages in cellular uptake and more safety in drug delivery and bio-imaging. Moreover, surfactant-coated NPs inhibited cellular uptake of NPs, and had selective toxicity to melanoma A875 cells rather than human umbilical vein endothelial cells (EVC-304), especially for surfactant polyoxyethylene octyl phenyl ether (Triton X-100) due to the generation of intracellular reactive oxygen species (ROS). The surfactant-free uniform NPs prepared from SORP, combining desirable characteristics of hydrophobic drugs and functional materials, may prove advantageous in simultaneous drug delivery, imaging and magnetic field manipulation applications.