Biomimetic coating of compound titania and hydroxyapatite on titanium.
Biomimetic coating of compound titania and hydroxyapatite on titanium.
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DOI：
10.1002/jbm.a.31401
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发表时间：
2007-12
期刊：
影响因子：
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通讯作者：
Lin Zhu;X. Ye;Guangxin Tang;N. Zhao;Y. Gong;Yuanli Zhao;Ji-zong Zhao;Xiufang Zhang
中科院分区：
文献类型：
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作者：
Lin Zhu;X. Ye;Guangxin Tang;N. Zhao;Y. Gong;Yuanli Zhao;Ji-zong Zhao;Xiufang Zhang
钛种植体表面改性是提高钛生物相容性的有效方法。本文描述了通过在钛表面应用二氧化钛和羟基磷灰石形成三层涂层来改善植入物生物相容性的努力。该三层涂层由作为顶层的HA（通过水热处理形成）、作为中间层的多孔TiO2（通过微弧氧化形成）和作为内层的致密TiO2膜（通过预阳极氧化形成）组成。评估了其物理化学特征、细胞行为和体内研究。采用扫描电镜观察、纤维连接蛋白和层粘连蛋白吸附、腐蚀试验和X射线衍射分析等方法研究了其理化性质。细胞行为学检测包括扫描电镜（SEM）形态学观察、MTT法计数和成骨样细胞碱性磷酸酶（ALP，成骨分化的代表酶）活性测定。在体内研究中，将标本包埋在颅骨创面中进行修复。通过实验分析，这种三层涂层的钛被证明具有优异的耐腐蚀性和良好的生物相容性，可以促进细胞增殖和骨形成。因此，这种改性钛是骨修复应用中未经处理的钛的改进替代品。
The modification on the titanium implant surface is an effective method to improve the biocompatibility of titanium. This article describes efforts to improve implant biocompatibility by applying titania and hydroxyapatite to form a three-layer coating on the titanium surface. This three-layer coating is made up of HA as the top layer (formed by hydrothermal treatment), porous TiO2 as the middle layer (formed by micro-arc oxidation) and a dense TiO2 film as the inner layer (formed by preanodic oxidation). The physicochemical characteristics, cell behavior and in vivo studies were assessed. The physicochemical characteristics were investigated using scanning electron micoscopy observation, fibronectin and laminin adsorption, corrosion test and X-ray diffraction analysis. Cell behavior included morphology observation with scanning electron microscopy (SEM), number count with methylthiazol tetrazolium (MTT) assay and Alkaline phosphatase (ALP, a representative enzyme of osteoblastic differentiation) activity of osteoblast-like MC3T3-E1 cells. In study in vivo the specimens were embedded in skull wound for repair. By the analysis of experiments, the titanium coated with this three-layer coating has been proved to have excellent corrosion resistance and good biocompatibility, which can promote cell proliferation and bone formation. So this modified titanium is an improved alternative to untreated titanium for bone repair applications.