Urban Focus of Rickettsia typhi and Rickettsia felis in Los Angeles, California
Urban Focus of Rickettsia typhi and Rickettsia felis in Los Angeles, California
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DOI：
10.1089/vbz.2010.0117
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发表时间：
2011-07-01
影响因子：
2.1
通讯作者：
Eremeeva, Marina E.
中科院分区：
文献类型：
--
作者：
Abramowicz, Kyle F.;Rood, Michael P.;Eremeeva, Marina E.
由伤寒立克次体引起的典型鼠伤寒是美国大陆德克萨斯州和南加州地区的地方病。我们在洛杉矶的一个市区进行了一次环境调查，该市区被确定为一例鼠伤寒的可能暴露地点。捕获4只严重感染印鼠客蚤的褐家鼠（平均每只动物32.5只跳蚤，范围20-42只），并收集跳蚤、血液和组织。对所有标本的DNA进行了R.伤寒沙门氏菌和猫立克次氏体，使用靶向立克次氏体柠檬酸合酶基因的TaqMan测定。虽然未检测到立克次体血症，但R.在每只大鼠的至少一个组织中检测到猫。3只大鼠的组织中也有R.伤寒DNA R. typhi和R.从每只动物收集的跳蚤中检测到猫DNA，平均最小感染率分别为10%和32.3%。虽然R.伤寒仍然以典型的东方跳蚤-鼠循环在洛杉矶市区传播，R.即使在这个协会中，猫也更普遍。
Classic murine typhus, caused by Rickettsia typhi, is endemic in the continental United States in areas of Texas and southern California. We conducted an environmental investigation in an urban area of Los Angeles identified as the probable exposure site for a case of murine typhus. Four Rattus norvegicus heavily infested with Xenopsylla cheopis (average 32.5 fleas per animal, range 20-42) were trapped, and fleas, blood, and tissues were collected. DNAs from all specimens were tested for R. typhi and Rickettsia felis using a TaqMan assay targeting the rickettsial citrate synthase gene. Although rickettsiemia was not detected, DNA of R. felis was detected in at least one tissue from each rat. Tissues from 3 rats were also positive for R. typhi DNA. R. typhi and R. felis DNAs were detected in fleas collected from each animal with average minimal infection rates of 10% and 32.3%, respectively. Although R. typhi still circulates in urban Los Angeles in the classic Oriental flea-rat cycle, R. felis is more prevalent, even in this association.