Polydatin improves vascular endothelial function by maintaining mitochondrial homeostasis under high glucose conditions.
Polydatin improves vascular endothelial function by maintaining mitochondrial homeostasis under high glucose conditions.
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DOI：
10.1038/s41598-023-43786-4
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发表时间：
2023-10-02
影响因子：
4.6
通讯作者：
Ma, Huijie
中科院分区：
文献类型：
--
作者：
Shah, Wahid;Zhao, Qiyue;Wang, Sen;Zhang, Miaomiao;Ma, Hongyu;Guan, Yue;Zhang, Yi;Liu, Yan;Zhu, Chunhua;Wang, Sheng;Zhang, Xiangjian;Dong, Jinghui;Ma, Huijie
先前的研究表明，聚胆碱（Poly）具有保护心脏的作用。然而，其潜在机制仍然难以捉摸。该研究表明，Poly（10µM）处理逆转了高糖（HG）诱导的乙酰胆碱引起的主动脉血管舒张的减少。聚乳酸还能改善乙酰胆碱诱导的糖尿病大鼠主动脉血管舒张。同时，Poly改善了HG条件下胸主动脉的形态损伤，提高了HUVECs的活力。此外，通过透射电镜、Western blotting和qPCR分析Poly在HG条件下的血管保护作用，发现Poly通过NLRP3/Caspase/1-IL-1β途径改善内皮细胞焦亡，增强动力蛋白相关蛋白1介导的线粒体分裂，增加HG条件下的线粒体膜电位。综上所述，聚乳酸可恢复乙酰胆碱诱导的HG培养后受损的血管舒张功能，这与减少氧化、炎症和焦亡、恢复线粒体膜电位和维持主动脉内皮细胞线粒体动态稳态有关。
Previous studies have shown that polydatin (Poly) confer cardioprotective effects. However, its underlying mechanisms remain elusive. This study showed that Poly (10 µM) treatment reversed the high glucose (HG)-induced decrease in acetylcholine-elicited vasodilation in aortas. Poly also improved the acetylcholine-induced vasodilation of aortic vessels isolated from diabetic rats. Meanwhile, Poly ameliorated the morphological damage of the thoracic aorta and improved the viability of HUVECs under HG conditions. Furthermore, analysis of the vasoprotective effect of Poly under HG conditions by transmission electron microscopy, Western blotting, and qPCR revealed that Poly improved endothelial pyroptosis through the NLRP3/Caspase/1-IL-1β pathway, enhanced dynamin-related protein 1-mediated mitochondrial fission, and increased the mitochondrial membrane potential under HG conditions. In conclusion, Poly restored acetylcholine-induced vasodilation impaired by HG incubation, which was associated with reduced oxidation, inflammation, and pyroptosis, the recovery of the mitochondrial membrane potential and maintenance of mitochondrial dynamic homeostasis of endothelial cells in the aortas.
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DOI：
10.1042/bj20081386
发表时间：
2009-01-01
期刊：
The Biochemical journal
影响因子：
--
作者：
Murphy MP
通讯作者：
Murphy MP
影响因子：
4
作者：
Chen H;Yu B;Guo X;Hua H;Cui F;Guan Y;Tian Y;Zhang X;Zhang Y;Ma H
通讯作者：
Ma H
影响因子：
5.5
作者：
Gioscia-Ryan, Rachel A.;LaRocca, Thomas J.;Seals, Douglas R.
通讯作者：
Seals, Douglas R.
高葡萄糖通过抑制单甲基转移酶 SETD8 表达诱导内皮 COX2 和 iNOS 表达
影响因子：
4.3
作者：
Qi, Jie;Wu, Qichao;Miao, Changhong
通讯作者：
Miao, Changhong
影响因子：
9.2
作者：
Li R;Li J;Huang Y;Li H;Yan S;Lin J;Chen Y;Wu L;Liu B;Wang G;Lan T
通讯作者：
Lan T