ANATOMICAL DISSEMINATION OF CIRCUMSPOROZOITE PROTEIN IN WILD AFROTROPICAL ANOPHELES AFFECTS MALARIA SPOROZOITE RATE DETERMINATION BY ELISA
ANATOMICAL DISSEMINATION OF CIRCUMSPOROZOITE PROTEIN IN WILD AFROTROPICAL ANOPHELES AFFECTS MALARIA SPOROZOITE RATE DETERMINATION BY ELISA
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DOI：
10.1111/j.1365-2915.1991.tb00524.x
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发表时间：
1991-01-01
影响因子：
1.9
通讯作者：
KOROS, JK
中科院分区：
文献类型：
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作者：
BEIER, JC;KOROS, JK
320只野生冈比亚按蚊和115只冈比亚按蚊的头部、胸部、翅膀、腿部和腹部。用酶联免疫吸附试验(EL ISA)检测恶性疟原虫韦尔奇疟原虫，以确定环子孢子(CS)蛋白的解剖扩散如何影响用ELISA法估计的疟疾子孢子率。在53例人体各部位均检出CS蛋白的按蚊中，头部阳性率为58.5%，胸部阳性率为67.0%，翅膀阳性率为39.6%，腿阳性率为52.8%，腹部阳性率为60.4%。平均吸光度最高的是胸部(1.17)，其次是头部(0.80)、腹部(0.67)、翅膀(0.48)和腿部(0.46)。11.3%(6/53)感染的按蚊体内有环子孢子蛋白存在于翅膀或腿部，而头部和胸部则没有。An-EL ISA感染率为12.8%(41/320)。仅在翅膀或腿上含有CS蛋白的6只蚊子，冈比亚亚种的感染率将增加到14.7%(47/320)。对传播的CS蛋白引起的感染蚊子比例的轻微高估，对现场采集的按蚊的相对感染率的估计几乎没有影响，因为在检测之前切除了腹部。然而，CS蛋白的广泛传播表明，对于没有腹部的蚊子，用ELISA法估计的子孢子载量可能不能充分测量唾液腺中的子孢子数量。在操作上，有必要对蚊子样本进行仔细处理，以通过酶联免疫吸附试验确定感染率，以防止代表个别蚊子的样本中混合翅膀或腿。
The head, thorax, wings, legs and abdomen of 320 wild-caught Anopheles gambiae Giles sensu lato and 115 An. funestus Giles were tested by an enzyme-linked immunosorbent assay (ELISA) for Plasmodium falciparum Welch to determine how anatomical dissemination of circumsporozoite (CS) protein could affect the estimation of malaria sporozoite rates by ELISA. Of fifty-three Anopheles with CS protein detected in any body part, positive reactions were observed for 58.5% of heads, 67.0% of thoraces, 39.6% of wings, 52.8% of legs and 60.4% of abdomens. Mean absorbance values (range 0-2.00) were highest in thorax samples (1.17), followed by heads (0.80), abdomens (0.67), wings (0.48) and legs (0.46).Circumsporozoite protein was present in the wings or legs, but not in the head or thorax, in 11.3% (6/53) of the infected Anopheles. The ELISA infection rate of 12.8% (41/320) for An. gambiae would have increased to 14.7% (47/320) by inclusion of six mosquitoes with CS protein in wings or legs alone. The slight overestimation of the proportion of infective mosquitoes due to disseminated CS protein would have little effect on estimates of relative infection rates by ELISA for field-collected Anopheles, with abdomens removed prior to testing. However, the widespread dissemination of CS protein indicates that sporozoite load estimates by ELISA, for mosquitoes without abdomens, may not provide adequate measurements of the numbers of sporozoites in the salivary glands. Operationally, careful processing of mosquito samples for the determination of infectively rates by ELISA is necessary to prevent the mixing of wings or legs among samples representing individual mosquitoes.