Cytochemical polarity in lateral geniculate interneurons.
Cytochemical polarity in lateral geniculate interneurons.
复制标题
外侧膝状体中间神经元的细胞化学极性。
DOI：
10.1016/0006-8993(94)91729-9
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发表时间：
1994
期刊：
影响因子：
2.9
通讯作者：
Dubin,MW
中科院分区：
文献类型：
--
作者：
Scheetz,AJ;Dubin,MW
为了确定突触前树突的细胞化学组成，我们已经研究了突触蛋白1，钙和钙调素依赖性蛋白激酶II（CaM-II），微管相关蛋白2（MAP-2）和血影蛋白在猫外侧膝状体（LGN）III类细胞的免疫电镜分布。特别注意这些中间神经元的树突，因为它们都是突触前和突触后的。树突状蛋白MAP-2和RBC血影蛋白在中间神经元树突中未观察到，但这些蛋白定位于中继细胞树突。突触囊泡相关蛋白synapsin 1存在于所有含有突触囊泡的轮廓中，包括树突状末梢。CaM-II，主要突触后密度蛋白，被发现在所有的树突。因此，LGN中间神经元树突隔室显示轴突和树突细胞化学性质。结果表明，独特的分子相互作用的可能性，在中间神经元树突终端。
To determine the cytochemical composition of presynaptic dendrites, we have examined the distribution of synapsin 1, calcium and calmodulin-dependent protein kinase II (CaM-II), microtubule-associated protein 2 (MAP-2) and spectrin in cat lateral geniculate (LGN) class III cells by immuno-EM. Special attention was paid to the dendrites of these interneurons because they are both pre- and postsynaptic. The dendritic proteins MAP-2 and RBC spectrin were not observed in interneuron dendrites but these proteins were localized in relay cell dendrites. The synaptic vesicle-associated protein synapsin 1 was present in all synaptic vesicle containing profiles, including dendritic terminals. CaM-II, the major postsyaptic density protein, was found in all dendrites. Thus, the LGN interneuron dendritic compartment displays both axonal and dendritic cytochemical properties. The results suggest the possibility of unique molecular interactions in interneuron dendritic terminals.