Constitutive and evoked release of ATP in adult mouse olfactory epithelium.
Constitutive and evoked release of ATP in adult mouse olfactory epithelium.
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DOI：
10.1515/biol-2022-0811
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发表时间：
2024
影响因子：
2.2
通讯作者：
中科院分区：
文献类型：
--
作者：
在成年嗅上皮（OE）中，ATP在恒定的细胞更新和损伤后神经再生中起作用。我们以前证明，组成和ATP诱发的ATP释放存在于新生小鼠OE和基础连续细胞翻转和损伤后神经再生，并参与了嘌呤能P2X7受体的激活诱发释放。我们假设这两种释放都存在于成年小鼠OE.To研究嗅觉感觉神经元ATP释放的假定贡献，我们使用了来自胚胎嗅基板细胞的嗅觉感觉神经元样OP6细胞。钙成像显示，OP6细胞和原代成人OE细胞培养表达功能性嘌呤受体。我们监测ATP释放OP6细胞和整个成人OE鼻甲HEK细胞作为生物传感器和荧光素酶测定。组成型ATP释放发生在OP6细胞和整个成年小鼠OE鼻甲，P2X7受体介导的诱发ATP释放只发生在鼻甲。本研究中所描述的ATP释放机制可能是成人OE中存在的恒定细胞翻转和损伤后神经再生的基础，因此，有必要对这些机制进行进一步研究，因为它将提高我们对OE组织稳态和损伤后再生的认识。
In adult olfactory epithelium (OE), ATP plays a role in constant cell turnover and post-injury neuroregeneration. We previously demonstrated that constitutive and ATP-evoked ATP release are present in neonatal mouse OE and underlie continuous cell turn-over and post-injury neuroregeneration, and that activation of purinergic P2X7 receptors is involved in the evoked release. We hypothesized that both releases are present in adult mouse OE. To study the putative contribution of olfactory sensory neurons to ATP release, we used olfactory sensory neuronal-like OP6 cells derived from the embryonic olfactory placode cells. Calcium imaging showed that OP6 cells and primary adult OE cell cultures express functional purinergic receptors. We monitored ATP release from OP6 cells and whole adult OE turbinates using HEK cells as biosensors and luciferin–luciferase assays. Constitutive ATP release occurs in OP6 cells and whole adult mouse OE turbinates, and P2X7 receptors mediated evoked ATP release occurs only in turbinates. The mechanisms of ATP release described in the present study might underlie the constant cell turn-over and post-injury neuroregeneration present in adult OE and thus, further studies of these mechanisms are warranted as it will improve our knowledge of OE tissue homeostasis and post-injury regeneration.
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影响因子：
3.5
作者：
Jia, Cuihong;Hegg, Colleen Cosgrove
通讯作者：
Hegg, Colleen Cosgrove
影响因子：
3.7
作者：
Jia C;Hayoz S;Hutch CR;Iqbal TR;Pooley AE;Hegg CC
通讯作者：
Hegg CC
影响因子：
3.6
作者：
Heron PM;Stromberg AJ;Breheny P;McClintock TS
通讯作者：
McClintock TS
影响因子：
4.2
作者：
Kanekar, Shami;Jia, Cuihong;Hegg, Colleen Cosgrove
通讯作者：
Hegg, Colleen Cosgrove