Developing testicular microvasculature in the golden hamster, Mesocricetus auratus: a model for angiogenesis under physiological conditions.
Developing testicular microvasculature in the golden hamster, Mesocricetus auratus: a model for angiogenesis under physiological conditions.
复制标题
金仓鼠睾丸微血管的发育，Mesocricetus auratus：生理条件下血管生成的模型。
DOI：
10.1159/000146982
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Bartke,A
中科院分区：
文献类型：
--
作者：
Mayerhofer,A;Bartke,A
观察了3、5、8、10、12、16、20、25、30和35日龄未成熟金黄地鼠睾丸微血管发育的超微结构，并与3月龄成年金黄地鼠的睾丸微血管进行了比较。此外，在注射辣根过氧化物酶(HRP)定位后，研究了16至35日龄仓鼠的血管通透性。在所有受检的未成熟仓鼠的睾丸中都出现了血管生成过程，并在8日龄至25日龄时最为明显。这些突起在3个月大仓鼠的睾丸中缺失。3d和5d，间质内可见少量内皮激活的未分化血管。内皮细胞从这些“母血管”开始迁移，并导致血管芽侵入管间间隙，然后管周间隙发生血管化(12天后)。发芽内皮细胞以基底膜的存在为特征，以丰富的细胞质和细胞器为特征。在发育中的血管中可见HRP阳性的裂隙，这些裂隙开放形成血管腔。HRP通过非特化的内皮接触和微吞饮小泡离开血管腔。到第16天，血-睾丸屏障阻止HRP进入基底室以外的生精小管。30日龄和35日龄时，大多数睾丸微血管为成熟型，3月龄时所有睾丸微血管均为成熟型，内皮细胞狭窄、不活跃，细胞间有特殊的接触(包括紧密连接)。这些结果表明，金黄地鼠睾丸的生后血管化是一个时序复杂的过程。由于高渗透性，血管萌发可能会影响睾丸的代谢状况，从而影响睾丸的成熟过程。在不同的病理和生理条件下，金黄地鼠睾丸的血管生成与其他器官和物种的血管生成过程具有典型的形态特征。因此，我们得出结论，出生后的睾丸可以被视为血管生成的生理模型。
The ultrastructure of the developing testicular microvasculature in the testes of immature (3, 5, 8, 10, 12, 16, 20, 25, 30 and 35 days old) golden hamsters was examined and compared to the testicular microvasculature of adult (3 months old) hamsters. In addition, in 16- to 35-day-old hamsters vascular permeability was studied after localization of injected horseradish peroxidase (HRP). Angiogenic processes were present in the testes of all examined immature hamsters and were most conspicuous between 8 and 25 days of age. These processes were absent in the testes of 3-month-old hamsters. On days 3 and 5, few undifferentiated blood vessels with activated endothelium were present in the interstitial spaces. Endothelial cell migration started from these ‘mother vessels’ and led to invasion of intertubular spaces by vascular sprouts, before vascularization of peritubular spaces occurred (after day 12). Sprouting endothelial cells were identified by the presence of a basal lamina and characterized by abundant cytoplasm and cell organelles. HRP-positive slits were seen in developing vessels, which opened to form the vascular lumen. HRP exited the vascular lumen through unspecialized endothelial contacts and micropinocytotic vesicles. By day 16, the blood-testis barrier prevented HRP from entering the seminiferous tubules beyond the basal compartment. By days 30 and 35 most testicular microvessels and at the age of 3 months all testicular microvessels were of the mature type, with narrow inactive endothelium and specialized cell contacts (including tight junctions). These results demonstrate that the postnatal vascularization of the testis in the golden hamster is a timed complex process. Due to high permeability, vascular sprouts are likely to influence the metabolic situation and thus the maturation processes of the testis. Angiogenesis in the golden hamster testis shares typical morphological features with angiogenic processes in other organs and species under various pathological and physiological conditions. We therefore conclude that the postnatal testis can be viewed as a physiological model of angiogenesis.