Overexpression of microRNA408 enhances photosynthesis, growth, and seed yield in diverse plants
Overexpression of microRNA408 enhances photosynthesis, growth, and seed yield in diverse plants
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DOI：
10.1111/jipb.12634
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发表时间：
2018-04-01
影响因子：
11.4
通讯作者：
Li, Lei
中科院分区：
文献类型：
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作者：
Pan, Jiawei;Huang, Dahui;Li, Lei
植物生产谷物、果实或饲料的能力最终取决于光合作用。然而，很少有人尝试研究microRNA，这是一类内源性小RNA转录后编程基因表达，与光合性状。我们专注于miR408，最保守的植物miRNAs之一，并在拟南芥，烟草和水稻中平行过表达。转基因植株都表现出叶绿体中铜含量增加，质体蓝素丰度升高，光合作用基因的诱导。通过气体交换和光学光谱分析，我们表明miR408的高表达通过提高辐射利用效率和二氧化碳固定能力来增强光合作用。因此，miR 408超积累植物表现出更高的营养生长速率。在所有三个过度生产miR408的物种中也观察到种子大小的扩大。此外，我们进行了为期2年的两个地点的田间试验，观察到miR408在水稻中的过表达显著增加了产量，这主要归因于粒重的增加。综上所述，这些结果表明，miR 408是光合作用的正调节因子，并且其基因工程是用于增强不同植物的光合性能和产量的有前途的途径。
The ability of a plant to produce grain, fruit, or forage depends ultimately on photosynthesis. There have been few attempts, however, to study microRNAs, which are a class of endogenous small RNAs post-transcriptionally programming gene expression, in relation to photosynthetic traits. We focused on miR408, one of the most conserved plant miRNAs, and overexpressed it in parallel in Arabidopsis, tobacco, and rice. The transgenic plants all exhibited increased copper content in the chloroplast, elevated abundance of plastocyanin, and an induction of photosynthetic genes. By means of gas exchange and optical spectroscopy analyses, we showed that higher expression of miR408 leads to enhanced photosynthesis through improving efficiency of irradiation utilization and the capacity for carbon dioxide fixation. Consequently, miR408 hyper-accumulating plants exhibited higher rate of vegetative growth. An enlargement of seed size was also observed in all three species overproducing miR408. Moreover, we conducted a 2-year-two-location field trial and observed miR408 overexpression in rice significantly increased yield, which was primarily attributed to an elevation in grain weight. Taken together, these results demonstrate that miR408 is a positive regulator of photosynthesis and that its genetic engineering is a promising route for enhancing photosynthetic performance and yield in diverse plants.