A novel CD44 v3 isoform is involved in head and neck squamous cell carcinoma progression
A novel CD44 v3 isoform is involved in head and neck squamous cell carcinoma progression
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DOI：
10.1067/mhn.2001.114674
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发表时间：
2001-04-01
影响因子：
3.4
通讯作者：
Bourguignon, LYW
中科院分区：
文献类型：
--
作者：
Franzmann, EJ;Weed, DT;Bourguignon, LYW
目的：CD44 包含参与肿瘤发生的同工型家族，在此我们研究 CD44 同工型在头颈鳞状细胞癌 (HNSCC) 进展中的作用。 材料和方法：HNSCC 标本进行逆转录酶聚合酶链反应 (RT-PCR)，然后进行 Southern 印迹分析。表面生物素化后，用 CD44 抗体沉淀 FaDu（下咽 HNSCC）和转染 CD44v3 的 COS-7 细胞，并通过蛋白质印迹分析进行比较。 FaDu 细胞进行双重免疫荧光染色和生长测定。结果：Southern 印迹分析表明肿瘤和正常组织中 CD44v3 亚型表达存在差异。克隆和测序揭示了 2 种新的 CD44v 亚型，Western blot 分析表明 COS-7 转染子和 FaDu 中存在 CD44v3 表达，双免疫荧光染色显示 FaDu 投影中 CD44v3 和肌动蛋白共定位，抗 CD44v3 抗体降低了 FaDu 生长。结论：HNSCC 组织和 FaDu 似乎表达 CD44v3 亚型，这些亚型可能促进肿瘤发生。意义：CD44v3 亚型可能是 HNSCC 治疗的有效肿瘤标志物和靶点。
OBJECTIVES: CD44 comprises a family of isoforms involved in tumorigenesis, Here we investigate the role of CD44 isoforms in head and neck squamous cell carcinoma (HNSCC) progression.MATERIALS AND METHODS: HNSCC specimens underwent reverse transcriptase-polymerase chain reaction (RT-PCR) followed by Southern blot analysis. After surface biotinylation, FaDu (hypopharyngeal HNSCC) and CD44v3-transfected COS-7 cells were CD44 antibody-precipitated and compared by Western blot analysis. FaDu cells underwent double immunofluorescence staining and growth assays.RESULTS: Southern blot analysis suggested differential CD44v3 isoform expression in tumor and normal tissue. Cloning and sequencing revealed 2 novel CD44v isoforms, Western blot analysis suggested CD44v3 expression in COS-7 transfectants and FaDu, Double immunofluorescence staining revealed colocalization of CD44v3 and actin in FaDu projections, Anti-CD44v3 antibody decreased FaDu growth.CONCLUSION: HNSCC tissue and FaDu appear to express CD44v3 isoforms, These isoforms may promote tumorigenesis.CLINICAL SIGNIFICANCE: CD44v3 isoforms may be effective tumor markers and targets for HNSCC therapy.