Syrian hamsters as a small animal model for SARS-CoV-2 infection and countermeasure development
Syrian hamsters as a small animal model for SARS-CoV-2 infection and countermeasure development
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DOI：
10.1073/pnas.2009799117
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发表时间：
2020-07-14
影响因子：
11.1
通讯作者：
Kawaoka, Yoshihiro
中科院分区：
文献类型：
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作者：
Imai, Masaki;Iwatsuki-Horimoto, Kiyoko;Kawaoka, Yoshihiro
2019年底，在武汉发现了一种新型冠状病毒(严重急性呼吸综合征冠状病毒2型；SARS-CoV-2)，该病毒在全球范围内迅速传播，对人类健康和全球经济造成了严重威胁。在这里，我们评估了SARS-CoV-2分离株在叙利亚仓鼠身上的复制能力和致病机理。SARS-CoV-2分离株在金黄地鼠肺内高效复制，经鼻感染后可引起严重的肺部病变。另外，CT图像显示严重的肺损伤具有SARS-CoV-2感染人肺的共同特征，包括严重的、双侧的、外周分布的、多小叶毛玻璃样阴影和肺实变区域。感染SARS-CoV-2的仓鼠产生中和抗体反应，并对随后的SARS-CoV-2再次攻击产生保护。此外，被动地将恢复期血清转移给幼龄仓鼠，即使在感染血清处理的仓鼠感染2d后给药，也能有效地抑制病毒在肺内的复制。总而言之，这些发现表明，这种叙利亚仓鼠模型将有助于了解SARS-CoV-2的发病机制，并测试疫苗和抗病毒药物。
At the end of 2019, a novel coronavirus (severe acute respiratory syndrome coronavirus 2; SARS-CoV-2) was detected in Wuhan, China, that spread rapidly around the world, with severe conse-quences for human health and the global economy. Here, we assessed the replicative ability and pathogenesis of SARS-CoV-2 isolates in Syrian hamsters. SARS-CoV-2 isolates replicated effi-ciently in the lungs of hamsters, causing severe pathological lung lesions following intranasal infection. In addition, microcomputed tomographic imaging revealed severe lung injury that shared char-acteristics with SARS-CoV-2-infected human lung, including severe, bilateral, peripherally distributed, multilobular ground glass opacity, and regions of lung consolidation. SARS-CoV-2-infected hamsters mounted neutralizing antibody responses and were protected against subsequent rechallenge with SARS-CoV-2. Moreover, passive transfer of convalescent serum to naive hamsters efficiently suppressed the replication of the virus in the lungs even when the serum was admin-istrated 2 d postinfection of the serum-treated hamsters. Collectively, these findings demonstrate that this Syrian hamster model will be useful for understanding SARS-CoV-2 pathogenesis and testing vaccines and antiviral drugs.