Calcium oxalate crystals in developing seeds of soybean
Calcium oxalate crystals in developing seeds of soybean
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DOI：
10.1006/anbo.2001.1453
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发表时间：
2001-08-01
期刊：
影响因子：
4.2
通讯作者：
Horner, HT
中科院分区：
文献类型：
--
作者：
Ilarslan, H;Palmer, RG;Horner, HT
发育中的大豆种子含有相对大量的草酸钙（CaOx）一水合物晶体。对Ca和CaOx的测试表明，Ca沉积物和晶体最初发生在珠柄中，其中单个维管束进入种子。晶体在珠被中形成，直到胚扩大到足以压碎内珠被的内部。晶体出现在发育中的子叶组织和胚轴中。所有晶体均在细胞液泡中形成。索中致密体和膜复合体明显。在内珠被中，细胞空泡呈现出未来晶体的形状。本文首次报道了大豆种子的这种假定的预定晶体霉菌。当每个组织中的晶体接近成熟时，每个晶体周围形成一个壁。这种细胞内晶体壁与细胞壁邻接。珠被晶体保持可见，直到扩大的胚压碎珠被;然后晶体消失。一项相关的研究表明，草酸干物质的最高百分比是在发育+16 d（受精后）的种子中达到的。在种子成熟后期下降。在+60 d时，CaOx的形成和消失是大豆种子发育的组成部分。我们的研究结果表明，钙存款和晶体功能作为钙存储的快速扩大胚胎。草酸盐可能通过一种或多种代谢途径参与种子贮藏蛋白的合成。(C)2001年《植物学年鉴》（Annals of Botany Company）
Young developing soybean seeds contain relatively large amounts of calcium oxalate (CaOx) monohydrate crystals. A test for Ca and CaOx indicated that Ca deposits and crystals initially occurred in the funiculus, where a single vascular bundle enters the seed. Crystals formed in the integuments until the embryo enlarged enough to crush the inner portion of the inner integument. Crystals then appeared in the developing cotyledon tissues and embryo axis. All crystals formed in cell vacuoles. Dense bodies and membrane complexes were evident in the funiculus. In the inner integument, cell vacuoles assumed the shape of the future crystals. This presumed predetermined crystal mould is reported here for the first time for soybean seeds, As crystals in each tissue near maturity, a wall forms around each crystal. This intracellular crystal wall becomes contiguous with the cell wall. Integument crystals remain visible until the enlarging embryo crushes the integuments; the crystals then disappear. A related study revealed that the highest percent of oxalate by dry mass was reached in the developing + 16 d (post-fertilization) seeds. and then decreased during late seed maturation. At +60 d, CaOx formation and disappearance are an integral part of developing soybean seeds. Our results suggest that Ca deposits and crystals functionally serve as Ca storage for the rapidly enlarging embryos. The oxalate, derived from one or more possible metabolic pathways, could be involved in see storage protein synthesis. (C) 2001 Annals of Botany Company.