Lethality of chlorine, chlorine dioxide, and a commercial fruit and vegetable sanitizer to vegetative cells and spores of Bacillus cereus and spores of Bacillus thuringiensis
Lethality of chlorine, chlorine dioxide, and a commercial fruit and vegetable sanitizer to vegetative cells and spores of Bacillus cereus and spores of Bacillus thuringiensis
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DOI：
10.1007/s10295-005-0212-7
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发表时间：
2005-07-01
影响因子：
3.4
通讯作者：
Scouten, AJ
中科院分区：
文献类型：
--
作者：
Beuchat, LR;Pettigrew, CA;Scouten, AJ
氯，二氧化氯（ClO 2），和商业原料水果和蔬菜消毒剂（适合粉末）的有效性进行了评估，在杀死蜡状芽孢杆菌的营养细胞和孢子和苏云金芽孢杆菌的孢子。最终目标是在研究中使用一种或两种菌种作为炭疽芽孢杆菌的潜在替代物，这些研究的重点是确定消毒剂在杀死食品接触表面和食品上的病原体方面的功效。用电化学技术产生的碱性（pH 10.5-11.0）ClO 2（200 mg/mL）处理减少了B的营养细胞的五种菌株混合物和孢子的五种菌株混合物的群体。蜡状芽孢杆菌在5 min内分别减少了5.4和6.4 log cfu/mL。这一发现与用200 mg/mL氯处理分别减少4.5和1.8 log cfu/mL相比。用Fit粉末产品的1.5%酸化（pH 3.0）溶液处理效果较差，导致B数量减少2.5-log和0.4-log cfu/mL。蜡状细胞和孢子。用碱性ClO 2（85 mg/mL）、酸化（pH 3.4）ClO 2（85 mg/mL）以及ClO 2（85 mg/mL）和Fit粉末产品（0.5%）（pH 3.5）的混合物处理，导致营养细胞/孢子种群分别减少5.3/5.6、5.3/5.7和5.31 6.0 log cfu/mL以上。治疗B。cereus和B.苏云金芽孢杆菌孢子在培养基中（3.4 mg/mL有机和无机固体），细胞在其中生长并产生具有等体积碱性的孢子（pH 12.1）二氧化氯（400 mg/mL）处理30 min后，菌群分别减少4.6和5.2 log cfu/mL，这表明在除孢子之外的材料存在下具有高致死性，所述材料可能与ClO 2的杀孢子活性反应并中和ClO 2的杀孢子活性。
Chlorine, chlorine dioxide (ClO2), and a commercial raw fruit and vegetable sanitizer (Fit powder) were evaluated for their effectiveness in killing vegetative cells and spores of Bacillus cereus and spores of Bacillus thuringiensis. The ultimate goal was to use one or both species as a potential surrogate(s) for Bacillus anthracis in studies that focus on determining the efficacy of sanitizers in killing the pathogen on food contact surfaces and foods. Treatment with alkaline (pH 10.5-11.0) ClO2 (200 mg/mL) produced by electrochemical technologies reduced populations of a five-strain mixture of vegetative cells and a five-strain mixture of spores of B. cereus by more than 5.4 and more than 6.4 log cfu/mL, respectively, within 5 min. This finding compares with respective reductions of 4.5 and 1.8 log cfu/mL resulting from treatment with 200 mg/mL chlorine. Treatment with a 1.5% acidified (pH 3.0) solution of Fit powder product was less effective, causing 2.5-log and 0.4-log cfu/mL reductions in the number of B. cereus cells and spores, respectively. Treatment with alkaline ClO2 (85 mg/mL), acidified (pH 3.4) ClO2 (85 mg/mL), and a mixture Of ClO2 (85 mg/mL) and Fit powder product (0.5%) (pH 3.5) caused reductions in vegetative cell/spore populations of more than 5.3/5.6, 5.3/5.7, and 5.31 6.0 log cfu/mL, respectively. Treatment of B. cereus and B. thuringiensis spores in a medium (3.4 mg/mL organic and inorganic solids) in which cells had grown and produced spores with an equal volume of alkaline (pH 12.1) ClO2 (400 mg/mL) for 30 min reduced populations by 4.6 and 5.2 log cfu/mL, respectively, indicating high lethality in the presence of materials other than spores that would potentially react with and neutralize the sporicidal activity Of ClO2.