Increased adenylyl cyclase type 1 mRNA, but not adenylyl cyclase type 2 in the rat hippocampus following antidepressant treatment
Increased adenylyl cyclase type 1 mRNA, but not adenylyl cyclase type 2 in the rat hippocampus following antidepressant treatment
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DOI：
10.1016/s0924-977x(99)00064-4
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发表时间：
2000-03-01
影响因子：
5.6
通讯作者：
Mork, A
中科院分区：
文献类型：
--
作者：
Jensen, JB;Mikkelsen, JD;Mork, A
腺苷酸环化酶（AC）系统受到几种类型的抗抑郁药治疗的影响，该系统中活性的增加与抗抑郁药的治疗作用有关。本研究比较了单剂量酸长期治疗与选择性5-羟色胺再摄取抑制剂西酞普兰（10 mg/kg，腹腔注射），对雄性Wistar大鼠脑AC系统的影响。此外，我们比较了长期西酞普兰和锂治疗对AC系统的影响。长期西酞普兰，而不是单剂量给药，增加了AC 1型mRNA在海马，而2型mRNA不受影响。长期锂治疗也增加了海马中的AC 1。然而，长期西酞普兰治疗没有增加AC 1型蛋白，基础或毛喉素刺激的AC活性，但CTP增加了海马AC活性。这可能表明增强的AC/G蛋白偶联。因此，西酞普兰可能通过作用于AC系统的组分而增加cAMP信号传导，而不影响AC 1型的蛋白水平。(C)2000 Elsevier Science B. V.保留所有权利。
The adenylyl cyclase (AC) system is affected by several types of antidepressant treatments, and increased activity in this system is linked to the therapeutic action of antidepressants. The present study was undertaken to compare the effects: of single-dose acid long-term treatment with the selective serotonin reuptake inhibitor, citalopram (10 mg/kg, i.p.), on the AC system in the male rat brain of Wistar rats. Furthermore, we compared the effects of long-term citalopram and lithium treatments on the AC system. Long-term citalopram, but not single-dose administration, increased the AC type 1 mRNA in the hippocampus, whereas type 2 mRNA was unaffected. Long-term lithium treatment also increased AC1 in the hippocampus. However, lone-term citalopram treatment did not increase AC type 1 protein, basal or forskolin-stimulated AC activity, but CTP increased AC activity in the hippocampus. This may indicate enhanced AC/G protein coupling. Thus, citalopram may increase cAMP signalling by acting on components of the AC system without affecting the protein level of the AC type 1. (C) 2000 Elsevier Science B.V. All rights reserved.