A new system for washing ion-exchange paper disks used in radiochemical enzyme assays.
A new system for washing ion-exchange paper disks used in radiochemical enzyme assays.
复制标题
一种用于清洗放射化学酶测定中使用的离子交换纸盘的新系统。
DOI：
10.1016/0003-2697(84)90237-9
复制
发表时间：
1984
影响因子：
2.9
通讯作者：
D. H. Ives
中科院分区：
文献类型：
--
作者：
D. H. Ives
一种新的装置已经开发出来，用于从离子交换纸盘上去除放射性底物，留下与纸结合的电离产物。它比旧的清洗离子交换纸的方法有许多重要的优点：（1）它更彻底和有效;未离子化材料的背景吸收降低到相对较低的水平。(2)易碎介质，如DEAE取代的纸（DE 81）不会受到搅拌器或搅拌器的侵蚀作用。(3)在整个样品应用和清洗过程中，盘保持有序，无需在清洗后对湿放射性盘进行耗时的分选。因此，可以方便地同时处理大量测定。(4)放射性核苷和其他可吸附基质被收集在一个药筒中，消除了对大量或过量放射性物质的处理和下沉处理。
A new device has been developed for the removal of radioactive substrate from ion-exchange paper disks, leaving ionized product bound to the paper. It has a number of important advantages over older methods of washing the ion-exchange paper: (1) It is much more thorough and efficient; background absorption of unionized material is reduced to relatively low levels. (2) Fragile media such as DEAE-substituted paper (DE81) are not subjected to the erosive effects of stirrers or agitators. (3) The disks remain organized throughout the sample application and washing procedure, eliminating need for time-consuming sorting of wet radioactive disks after washing. A larger number of assays can therefore be processed conveniently at one time. (4) Radioactive nucleosides and other adsorbable substrates are collected in a cartridge, eliminating the handling and sinkdisposal of large volumes or excessive quantities of radioactive materials.
来自大麦的核苷磷酸转移酶。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Prasher,DC;Carr,MC;Ives,DH;Tsai,TC;Frey,PA
通讯作者：
Frey,PA