Hyaluronic Acid-Quantum Dot Conjugates for In Vivo Lymphatic Vessel Imaging
Hyaluronic Acid-Quantum Dot Conjugates for In Vivo Lymphatic Vessel Imaging
复制标题
DOI：
10.1021/nn900138d
复制
发表时间：
2009-06-01
期刊：
影响因子：
17.1
通讯作者：
Kim, Sungjee
中科院分区：
文献类型：
--
作者：
Bhang, Suk Ho;Won, Nayoun;Kim, Sungjee
报道了一种简单而新颖的静电偶联方法，该方法提供了一种胶体稳定且尺寸在50 - 120 nm范围内可调的透明质酸-量子点偶联物（HA-QD）。HA-QD显示出癌症靶向效率，这表明了诊断和成像应用。该偶联物还被证明具有体外和体内淋巴管荧光染色能力。在小动物模型中使用HA-QD，淋巴管在体内实时可视化数天。对结合物和未结合的对应物进行全面的细胞毒性评价。HA-QD展示了癌症成像和淋巴管变化（如淋巴管生成）实时可视化的潜力。
A simple and novel electrostatic coupling method is reported, which provides a hyaluronic acid-quantum dot conjugate (HA-QD) that is colloidally stable and size-tunable from 50 to 120 nm. The HA-QDs show cancer targeting efficiency, which suggests diagnostic and imaging applications. The conjugates are also demonstrated for the fluorescence staining capability for lymphatic vessels in vitro and in vivo. Using the HA-QDs in a small animal model, lymphatic vessels are visualized real-time in vivo for days. Comprehensive cytotoxicity evaluations are made for the conjugates and the unconjugated counterpart. The HA-QDs showcase the potentials toward cancer imaging and real-time visualization of changes in lymphatic vessels such as lymphangiogenesis.