Combinational Antibacterial Activity of Nisin and 3-Phenyllactic Acid and Their Co-production by Engineered Lactococcus lactis.
Combinational Antibacterial Activity of Nisin and 3-Phenyllactic Acid and Their Co-production by Engineered Lactococcus lactis.
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乳链菌肽与3-苯基乳酸的联合抗菌活性及其工程化乳酸乳球菌的联产
DOI：
10.3389/fbioe.2021.612105
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发表时间：
2021
影响因子：
5.7
通讯作者：
Qiao J
中科院分区：
文献类型：
--
作者：
Liu J;Huang R;Song Q;Xiong H;Ma J;Xia R;Qiao J
乳链菌肽是由乳酸乳球菌产生的一种新型抗菌素，具有良好的抗菌活性和食品安全性，广泛应用于肉类和乳制品行业。研究表明，与其他抗菌剂联用可增强其抗菌活性。在这项研究中，我们证明了乳链菌肽和3-苯基乳酸（PLA）的组合显示出良好的联合抗菌活性，对食源性致病菌，包括沙门氏菌。xylosus和M.黄体。通过对肉、奶贮藏过程中的微生物分析和草莓腐烂率的测定，进一步验证了该复合材料在食品保鲜中的应用潜力。扫描电镜观察显示乳酸链球菌素与聚乳酸的协同作用模式不同，可能是乳酸链球菌素与聚乳酸协同作用的靶向细胞。基于以上结果，以食品级乳酸链球菌L. lactis F44，乳链菌肽Z生产者。通过敲除两个L-乳酸脱氢酶（LDH）和过表达D-LDHY 25 A，PLA的产量与野生型相比显著增加了1.77倍。抑菌试验表明，重组菌发酵产物具有高效的抑菌活性。这些结果为乳酸链球菌素-聚乳酸共表达乳酸杆菌提供了良好的前景。乳酸菌具有显著的抗菌活性和成本效益，因此在食品防腐中具有重要的应用价值。
Nisin produced by certain Lactococcus lactis strains is commercially used in meat and dairy industries because of its effective antibacterial activity and food safety characteristics. It has been proved that the antibacterial activity could be enhanced when combined with other antimicrobial agents. In this study, we demonstrated that nisin and 3-phenyllactic acid (PLA) in combination displayed excellent combinational antibacterial activity against foodborne pathogens including S. xylosus and M. luteus. The potential application in food preservation was further verified via microbial analysis during the storage of meat and milk, and determination of strawberry rot rate. Scanning electron microscopy observation indicated a distinct mode of PLA with nisin, which may target at the dividing cell, contributing to their combinational antibacterial effect of nisin and PLA. Considering the positive results, a nisin-PLA co-producing strain was constructed based on the food-grade strain L. lactis F44, a nisin Z producer. By the knockout of two L-lactate dehydrogenase (LDH) and overexpression of D-LDHY25A, the yield of PLA was significantly increased 1.77-fold in comparison with the wild type. Anti-bacterial assays demonstrated that the fermentation product of the recombinant strain performed highly effective antibacterial activity. These results provided a promising prospect for the nisin-PLA co-expressing L. lactis in food preservation on account of its considerable antibacterial activity and cost-effective performance.
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影响因子：
4.5
作者：
Bauer, AK;Dwyer-Nield, LD;Malkinson, AM
通讯作者：
Malkinson, AM
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影响因子：
6.1
作者：
Li, Xingfeng;Jiang, Bo;Zhang, Tao
通讯作者：
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影响因子：
5
作者：
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通讯作者：
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影响因子：
5.2
作者：
Liu, Jiaheng;Meng, Furong;Qiao, Jianjun
通讯作者：
Qiao, Jianjun
影响因子：
3.6
作者：
Perez-Nunez, Daniel;Briandet, Romain;Guedon, Eric
通讯作者：
Guedon, Eric