Separating vesicle fusion and exocytosis in hypertonic conditions.
Separating vesicle fusion and exocytosis in hypertonic conditions.
复制标题
在高渗条件下分离囊泡融合和胞吐作用。
DOI：
10.1111/j.1749-6632.2002.tb04470.x
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发表时间：
2002
影响因子：
5.2
通讯作者：
Wightman,RMark
中科院分区：
文献类型：
--
作者：
Troyer,KevinP;Mundorf,MichelleL;Fries,HarveyE;Wightman,RMark
翻译后摘要：嗜铬细胞和肥大细胞的囊泡内容物和细胞外液之间的渗透梯度的存在在胞吐过程中起着关键作用。通过提高外部缓冲液的渗透压来改变该梯度，允许抑制来自细胞的胞吐作用，并使用循环伏安法在刺激时分离和鉴定峰前电流事件。随后的渗透梯度置换导致内容物从融合的囊泡中释放。
Abstract:The existence of an osmotic gradient between the vesicular contents of chromaffin and mast cells and the extracellular fluid plays a key role in the exocytotic process. Altering this gradient by elevating the external buffer osmolarity allows for the inhibition of exocytosis from cells and isolation and identification of prespike current events upon stimulation using cyclic voltammetry. Subsequent replacement of the osmotic gradient leads to release of the contents from fused vesicles.