Characterization of the immunophenotype of the tumor budding and its prognostic implications in squamous cell carcinoma of the lung
Characterization of the immunophenotype of the tumor budding and its prognostic implications in squamous cell carcinoma of the lung
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DOI：
10.1016/j.lungcan.2011.11.010
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发表时间：
2012-06-01
期刊：
影响因子：
5.3
通讯作者：
Ochiai, Atsushi
中科院分区：
文献类型：
--
作者：
Taira, Tetsuhiko;Ishii, Genichiro;Ochiai, Atsushi
肿瘤出芽在形态学上定义为小簇癌细胞的浸润。虽然已经阐明了腺癌中出芽细胞的生物学特性（粘附分子和分化标志物的表达降低），但是肺的鳞状细胞癌（SqCC）中的细胞的生物学特性仍有待澄清。我们研究了217例肺SqCC患者的临床病理资料。此外，我们评估了芽殖细胞的免疫组织化学特性。83例（38.2%）患者观察到肿瘤出芽。有肿瘤芽生者与无肿瘤芽生者的5年生存率差异有统计学意义（45.6% vs.64.0%，p < 0.001）。与形成固体巢的癌细胞相比，出芽细胞（BC）表现出细胞粘附分子（E-钙粘蛋白：p = 0.004，β-连环蛋白; p = 0.002）的表达水平降低和层粘连蛋白-5 γ 2（p = 0.001）的表达水平增加。另一方面，在分化标志物（p63和podoplanin）的染色评分中没有发现BC和形成巢的癌细胞之间的显著差异。多变量分析显示，肿瘤出芽是肺SqCC患者的重要独立预后因素（p = 0.022）。肿瘤出芽是肺SqCC患者的独立不良预后因素。虽然SqCC中的出芽细胞表现出细胞粘附分子的表达水平降低，但特异性分化标志物的表达水平被保留，这表明SqCC中的出芽机制可能至少部分不同于腺癌中的出芽机制。(c)2011爱思唯尔爱尔兰有限公司保留所有权利。
Tumor budding is morphologically defined as infiltration by small clusters of cancer cells. While the biological properties of budding cells in adenocarcinoma (decreased expression of adhesion molecules and of differentiation markers) have been elucidated, those of the cells in squamous cell carcinoma (SqCC) of the lung still remain to be clarified. We examined the clinicopathological data of 217 patients with SqCC of the lung. Furthermore we evaluated the immunohistochemical properties of the budding cells. Tumor budding was observed in 83 (38.2%) patients. A statistically significant difference was observed in overall 5-year survival rates between the cases showing tumor budding and the cases not showing budding (45.6% vs. 64.0%, p < 0.001). As compared with cancer cells forming solid nests, budding cells (BCs) exhibited reduced expression levels of the cellular adhesion molecules (E-cadherin: p = 0.004, beta-catenin; p = 0.002) and increased expression levels of laminin-5 gamma 2 (p = 0.001). On the other hand, no significant differences in the staining scores for differentiation markers (p63 and podoplanin) were found between BCs and cancer cells forming nests. Multivariate analysis revealed that tumor budding was a significant independent prognostic factor in patients with SqCC of the lung (p = 0.022). Tumor budding is an independent adverse prognostic factor in patients with SqCC of the lung. Although budding cells in SqCC exhibited reduced expression levels of the cellular adhesion molecules, the expression levels of specific differentiation markers were retained, suggesting that the budding mechanism in SqCC may differ, at least in part, from that in adenocarcinoma. (c) 2011 Elsevier Ireland Ltd. All rights reserved.