Sperm MMP-2 is indispensable for fast electrical block to polyspermy at fertilization in Xenopus tropicalis
Sperm MMP-2 is indispensable for fast electrical block to polyspermy at fertilization in Xenopus tropicalis
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DOI：
10.1002/mrd.23540
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发表时间：
2021-10-07
影响因子：
2.5
通讯作者：
Iwao，Yasuhiro
中科院分区：
文献类型：
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作者：
Watabe，Mami;Hiraiwa，Azusa;Iwao，Yasuhiro
精子基质金属蛋白酶-2(MMP2)是青蛙受精所必需的。单精受精是由正的受精潜力所介导的对多精受精的快速的电子阻断来确保的。为了确定MMP2血凝蛋白结构域(HPx)在非洲爪蛙受精过程中快速阻断多精受精的作用，我们利用转录激活因子样效应子核酸酶方法制备了缺失Δ基因的突变青蛙。MMP2 MMP2 HPX(−/−)精子能够受精野生型(WT；+/+)卵。但也有部分卵子发生了多精受精。突变精子产生的正常受精势为10 mV，并能在10 mV下受精，而WT精子在此水平上从未受精。突变精子在电压依赖受精过程中的敏感度降低。这项研究首次证明了精子中基质金属蛋白酶-2分子的遗传变化导致了单精子物种受精过程中的多精受精。我们的发现提供了可靠的证据，证明精子基质金属蛋白酶-2在异种受精过程中对多精子的快速电阻断是必不可少的。
Sperm matrix metalloproteinase‐2 (MMP‐2) is necessary for frog fertilization. Monospermy is ensured by a fast, electrical block to polyspermy mediated by a positive fertilization potential. To determine the role of the MMP‐2 hemopexin domain (HPX) in a fast block to polyspermy during fertilization of the frog,Xenopus tropicalis, we prepared mutant frogs deficient inmmp2gene using the transcription activator‐like effector nuclease method.mmp2ΔHPX (−/−) sperm without MMP‐2 protein were able to fertilize wild‐type (WT; +/+) eggs. However, polyspermy occurred in some eggs. The mutant sperm generated a normal fertilization potential amounting to 10 mV, and were able to fertilize eggs at 10 mV, at which WT sperm never fertilized. Sensitivity during voltage‐dependent fertilization decreased in mutant sperm. This study demonstrates for the first time that the genetic alteration of the MMP‐2 molecule in sperm causes polyspermy during fertilization of a monospermic species. Our findings provide reliable evidence that sperm MMP‐2 is indispensable for the fast, electrical block to polyspermy duringXenopusfertilization.