Tracking B cell responses to the SARS-CoV-2 mRNA-1273 vaccine.
Tracking B cell responses to the SARS-CoV-2 mRNA-1273 vaccine.
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DOI：
10.1016/j.celrep.2023.112780
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发表时间：
2023-07-25
期刊：
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
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作者：
疫苗接种后的保护性免疫是由长寿的抗体分泌细胞和静止的记忆B细胞(MBCs)维持的。通过多模式单细胞分析，纵向评估了三名感染ı伊夫的人对两剂SARS-CoV-2mRNA-1273疫苗的反应。分离的成浆细胞和SPEKE+(S-2P+)和S-2P−B细胞的表面蛋白、转录组和B细胞受体的综合分析表明S-2P+细胞之间存在克隆性扩增和累积突变。这些细胞富含在一簇表达免疫球蛋白G的MBCs中，并沿着植根于CXCR3+MBCs的分叉轨迹进化。在12个进化的S-2P+克隆中，有几个克隆早期为浆母细胞，后期为CD71+活化和静止的多核细胞，并表现为队列内和/或队列间的BCR收敛。这些关系表明了SARS-CoV-2疫苗产生的多核细胞的协调和可预测的进化。使用多组单细胞分析，Assis等人。显示了一条涉及浆母细胞和激活的和静止的记忆B细胞的协调轨迹，以响应最初的SARS-CoV-2mRNA疫苗。SPEK特异性BCR曲目分析显示，在6个月的研究期间，亲和力逐渐成熟，并揭示了研究参与者和其他队列中趋同的证据。
Protective immunity following vaccination is sustained by long-lived antibody-secreting cells and resting memory B cells (MBCs). Responses to two-dose SARS-CoV-2 mRNA-1273 vaccination are evaluated longitudinally by multimodal single-cell analysis in three infection-naıïve individuals. Integrated surface protein, transcriptomics, and B cell receptor (BCR) repertoire analysis of sorted plasmablasts and spike+ (S-2P+) and S-2P− B cells reveal clonal expansion and accumulating mutations among S-2P+ cells. These cells are enriched in a cluster of immunoglobulin G-expressing MBCs and evolve along a bifurcated trajectory rooted in CXCR3+ MBCs. One branch leads to CD11c+ atypical MBCs while the other develops from CD71+ activated precursors to resting MBCs, the dominant population at month 6. Among 12 evolving S-2P+ clones, several are populated with plasmablasts at early timepoints as well as CD71+ activated and resting MBCs at later timepoints, and display intra- and/or inter-cohort BCR convergence. These relationships suggest a coordinated and predictable evolution of SARS-CoV-2 vaccine-generated MBCs. Using multiomic single-cell analyses, Assis et al. show a coordinated trajectory involving plasmablasts and activated and resting memory B cells in response to primary SARS-CoV-2 mRNA vaccination. Spike-specific BCR repertoire analysis shows incremental affinity maturation across the 6-month study period and reveals evidence of convergence among study participants and other cohorts.
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影响因子：
64.8
作者：
Cao, Junyue;Spielmann, Malte;Shendure, Jay
通讯作者：
Shendure, Jay
影响因子：
32.4
作者：
DeWolf, Susan;Laracy, Justin C.;Perales, Miguel-Angel;Kamboj, Mini;van den Brink, Marcel R. M.;Vardhana, Santosha
通讯作者：
Vardhana, Santosha
mRNA疫苗会诱导SARS-COV-2和关注的变体诱导耐用的免疫记忆。
DOI：
10.1126/science.abm0829
发表时间：
2021-12-03
期刊：
Science (New York, N.Y.)
影响因子：
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作者：
通讯作者：
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DOI：
10.1056/nejmoa2035389
发表时间：
2021-02-04
期刊：
The New England journal of medicine
影响因子：
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作者：
Baden LR;El Sahly HM;Essink B;Kotloff K;Frey S;Novak R;Diemert D;Spector SA;Rouphael N;Creech CB;McGettigan J;Khetan S;Segall N;Solis J;Brosz A;Fierro C;Schwartz H;Neuzil K;Corey L;Gilbert P;Janes H;Follmann D;Marovich M;Mascola J;Polakowski L;Ledgerwood J;Graham BS;Bennett H;Pajon R;Knightly C;Leav B;Deng W;Zhou H;Han S;Ivarsson M;Miller J;Zaks T;COVE Study Group
通讯作者：
COVE Study Group
DOI：
10.1126/science.abm3425
发表时间：
2022-01-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
通讯作者：
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