Development of automated paper-based devices for sequential multistep sandwich enzyme-linked immunosorbent assays using inkjet printing
Development of automated paper-based devices for sequential multistep sandwich enzyme-linked immunosorbent assays using inkjet printing
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DOI：
10.1039/c2lc40690j
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发表时间：
2013-01-01
期刊：
影响因子：
6.1
通讯作者：
Takamura, Yuzuru
中科院分区：
文献类型：
--
作者：
Apilux, Amara;Ukita, Yoshiaki;Takamura, Yuzuru
据我们所知，这是第一份报告的纸基设备自动化的顺序多步骤程序的酶联免疫吸附测定（ELISA），只需要一个单一的步骤应用的样品溶液。该设备基于一片具有特殊设计通道的硝酸纤维素（NC）膜，其中所有试剂都应用于不同位置，以控制流体行进到检测区域。喷墨印刷方法是一种简单且低成本的方法，用于创建流动通道和器件屏障图案。发现所制造的屏障是液体沿着NC膜中的印刷设计的有效边界，使得能够直接控制试剂流动时间。ELISA结果通过单步样品应用获得。所开发的装置（所谓的自动纸基装置）为夹心ELISA提供了简单的程序，同时减少了测定时间和试剂消耗。使用具有软件处理的数码相机成像测量比色结果。本文开发的方法的能力被成功地用于通过ELISA测定人绒毛膜促性腺激素（hCG）的水平。
To the best of our knowledge, this is the first report on paper-based devices for automating the sequential multistep procedures of a sandwich-type enzyme-linked immunosorbent assay (ELISA) that require only a single-step application of the sample solution. The device was based on a piece of nitrocellulose (NC) membrane with specially designed channels, where all the reagents are applied at different locations in order to control the fluid travel to the detection region. The inkjet printing method, a simple and low-cost process, was used to create the flow channel and device barrier patterns. The fabricated barrier was found to be an efficient boundary for the liquid along the printed design in the NC membrane, enabling direct control of the reagent flow time. ELISA results were obtained with a single-step sample application. The developed devices (so-called automated paper-based devices) provided a simple procedure for the sandwich ELISA, while reducing assay time and reagent consumption. Colorimetric results were measured using digital camera imaging with software processing. The capability of the method developed herein was successfully used to determine the levels of human chorionic gonadotropin (hCG) by ELISA.