Use of Localized Reconstruction to Visualize the Shigella Phage Sf6 Tail Apparatus.
Use of Localized Reconstruction to Visualize the Shigella Phage Sf6 Tail Apparatus.
复制标题
使用局部重建来可视化志贺氏菌噬菌体 Sf6 尾部装置。
DOI：
10.1007/978-1-0716-3549-0_14
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Cingolani,Gino
中科院分区：
文献类型：
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作者：
Hou,Chun-FengDavid;Li,Fenglin;Iglesias,Stephano;Cingolani,Gino
低温电子显微镜（cryo-EM）单粒子分析已经彻底改变了二十面体病毒的结构分析，包括尾噬菌体。近年来，局部（或聚焦）重建已成为一种强大的数据分析方法，以捕获对称性失配和解决二十面体病毒的不对称特征。在这里，我们描述的方法来重建的2.65-MDa尾器的志贺氏噬菌体Sf 6，一个代表性的成员，thePodoviridaesophagia家庭。
Cryogenic electron microscopy (cryo-EM) single-particle analysis has revolutionized the structural analysis of icosahedral viruses, including tailed bacteriophages. In recent years, localized (or focused) reconstruction has emerged as a powerful data analysis method to capture symmetry mismatches and resolve asymmetric features in icosahedral viruses. Here, we describe the methods used to reconstruct the 2.65-MDa tail apparatus of theShigellaphage Sf6, a representative member of thePodoviridaesuperfamily.