Integration of multicopy extrachromosomal transgenes into defined loci without phenotypes.

Integration of multicopy extrachromosomal transgenes into defined loci without phenotypes.
复制标题

DOI:
10.17912/micropub.biology.000571
复制
发表时间:
2022
影响因子:
--
通讯作者:
Mitani, Shohei
Mitani, Shohei
中科院分区:
其他
文献类型:
--
作者:
Yoshina, Sawako;Mitani, Shohei

文献摘要

相似文献

我们展示了如何使用CRISPR/Cas9系统,推测非表型基因座可用于多拷贝染色体外转基因的整合位点。我们使用了四个基因座,在我们的手中没有表现出明显的表型,作为任何其他基因座没有表型的模型。
We show how presumably non-phenotypic loci can be used for integration sites of multi-copy extrachromosomal transgenes, using the CRISPR/Cas9 system. We used four loci, which show no apparent phenotype in our hands, as a model for any other loci with no phenotype.