Integration of multicopy extrachromosomal transgenes into defined loci without phenotypes.
Integration of multicopy extrachromosomal transgenes into defined loci without phenotypes.
复制标题
DOI:
10.17912/micropub.biology.000571
复制
发表时间:
2022
影响因子:
--
通讯作者:
Mitani, Shohei
中科院分区:
文献类型:
--
作者:
Yoshina, Sawako;Mitani, Shohei
We show how presumably non-phenotypic loci can be used for integration sites of multi-copy extrachromosomal transgenes, using the CRISPR/Cas9 system. We used four loci, which show no apparent phenotype in our hands, as a model for any other loci with no phenotype.