Catellibacterium nanjingense sp. nov., a propanil-degrading bacterium isolated from activated sludge, and emended description of the genus Catellibacterium.
Catellibacterium nanjingense sp. nov., a propanil-degrading bacterium isolated from activated sludge, and emended description of the genus Catellibacterium.
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DOI：
10.1099/ijs.0.029819-0
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发表时间：
2012-03
影响因子：
2.8
通讯作者：
Jun Zhang;Shu-An Chen;Jin-Wei Zheng;Shu Cai;Bao-jian Hang;Jian He;Shun-Peng Li
中科院分区：
文献类型：
--
作者：
Jun Zhang;Shu-An Chen;Jin-Wei Zheng;Shu Cai;Bao-jian Hang;Jian He;Shun-Peng Li
从污水生物处理设施的活性污泥中分离到一株兼性厌氧、无芽胞、不能运动、过氧化氢酶和氧化酶阳性、革兰氏阴性杆菌和杆状细菌，命名为Y12(T)。该菌株能在3d内降解约90%的添加丙醇(100mgL(-1))。生长条件为0-4.5%(w/v)的氯化钠(最适0.5%)、10-40℃(最适28℃)和pH 5.5-10.0(最适pH 7.0)。未观察到囊泡内膜结构和光异养生长。主要呼吸对苯二酚为泛醌-10，细胞内主要脂肪酸为特征8(C(18：1)ω6c和/或C(18：1)ω7c)。菌株Y12(T)的基因组DNA G+C含量为63.7mol%。基于16S rRNA基因序列比较的系统发育分析表明，菌株Y12(T)与卡氏链杆菌DCA-1(T)的序列相似性最高(99.1%)，与链杆菌属其他物种的相似性为96.0%，是链杆菌属的一个成员。菌株Y12(T)与卡氏梭菌DCA-1(T)的DNA-DNA亲缘关系值较低。根据表型、基因分型和系统发育特征，菌株Y12(T)是链状杆菌属的一个新种，命名为南京链杆菌属(Catelliba nanjingense sp.)11月是提议的。模式菌株为Y12(T)(=CCTCC AB 2010218(T)=KCTC 23298(T))。并对链状杆菌属进行了订正描述。
A novel facultatively anaerobic, non-spore-forming, non-motile, catalase- and oxidase-positive, Gram-negative and rod-shaped bacterial strain, designated Y12(T), was isolated from activated sludge of a wastewater bio-treatment facility. The strain was able to degrade about 90% of added propanil (100 mg l(-1)) within 3 days of incubation. Growth occurred in the presence of 0-4.5% (w/v) NaCl (optimum 0.5%), at 10-40 °C (optimum 28 °C) and at pH 5.5-10.0 (optimum pH 7.0). Vesicular internal membrane structures and photoheterotrophic growth were not observed. The major respiratory quinone was ubiquinone-10 and the major cellular fatty acid was summed feature 8 (C(18:1)ω6c and/or C(18:1)ω7c). The genomic DNA G+C content of strain Y12(T) was 63.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain Y12(T) was a member of the genus Catellibacterium, as it showed highest sequence similarities to Catellibacterium caeni DCA-1(T) (99.1%) and <96.0% similarities with other species of the genus Catellibacterium. Strain Y12(T) showed low DNA-DNA relatedness values with C. caeni DCA-1(T). Based on phenotypic, genotypic and phylogenetic properties, strain Y12(T) represents a novel species of the genus Catellibacterium, for which the name Catellibacterium nanjingense sp. nov. is proposed. The type strain is Y12(T) (=CCTCC AB 2010218(T) =KCTC 23298(T)). An emended description of the genus Catellibacterium is also presented.