Biologic and molecular characterization of Toxoplasma gondii isolates from pigs from Portugal
Biologic and molecular characterization of Toxoplasma gondii isolates from pigs from Portugal
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DOI：
10.1016/j.vetpar.2005.08.012
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发表时间：
2006-01-30
影响因子：
2.6
通讯作者：
Dubey, JP
中科院分区：
文献类型：
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作者：
de Sousa, S;Ajzenberg, D;Dubey, JP
在葡萄牙，对动物弓形虫感染知之甚少。在本文中，我们报告的第一个分离的活弓形虫在葡萄牙的猪。用改良凝集试验（MAT）检测333头猪，血清稀释度为1：20时，52头（15.6%）猪在屠宰前检出弓形虫抗体。对37头血清学阳性猪进行了弓形虫分离试验。在酸性胃蛋白酶中消化每头猪的脑和/或心脏样品，并对小鼠进行生物测定。从15头猪中分离到活弓形虫。对SAG 2位点的PCR产物进行限制性片段长度多态性分析和微卫星分析，结果表明II型为11型，4株为III型。结果表明，弓形虫在表型和遗传学上与Elsevier B. V.发表的美国猪分离株相似。
Little is known of Toxoplasma gondii infections in animals in Portugal. In the present paper, we report the first isolation of viable T gondii from pigs in Portugal. Antibodies to T gondii were found in 52 (15.6%) of 333 pigs prior to slaughter using the modified agglutination test (MAT) at a serum dilution of 1:20. Attempts were made to isolate T gondii from 37 seropositive pigs. Samples of brain and/or heart from each pig were digested in acid pepsin, and bioassayed into mice. Viable T gondii was isolated from 15 pigs. Restriction fragment length polymorphism on products of SAG2 locus amplified by PCR and microsatellite analysis revealed that II isolates were Type 11 and four were Type III. The results indicate that phenotypically and genetically T gondii are similar to isolates from pigs from the U.S. Published by Elsevier B.V.