Telomere Length of Human Adult Bronchial Epithelium and Bronchogenic Squamous Cell Carcinoma Measured Using Tissue Quantitative Fluorescence in situ Hybridization.
Telomere Length of Human Adult Bronchial Epithelium and Bronchogenic Squamous Cell Carcinoma Measured Using Tissue Quantitative Fluorescence in situ Hybridization.
复制标题
使用组织定量荧光原位杂交测量人类成人支气管上皮和支气管鳞状细胞癌的端粒长度。
作者：
Aida S;Aida J;Hasegawa K;Kumasaka T;Shimazaki H;Tamai S;Takubo K.
背景端粒是位于染色体末端的重复DNA序列。众所周知，端粒功能障碍导致的染色体和基因组不稳定性在某些器官的癌变中起着重要作用。目的本研究的目的是检查吸烟与患有和未患有肺癌的个体中人支气管上皮细胞端粒长度之间的相关性。患者和方法我们检查了 68 例非肺癌成人尸检病例和 24 例手术切除的肺鳞状细胞癌病例。采用组织定量荧光原位杂交法测量支气管上皮基底细胞的端粒长度，并以归一化端粒与着丝粒比率（NTCR）表示。结果尸检个体包括27名当前吸烟者（CuS）、33名从不吸烟者（NeS）和8名戒烟者（ExS）。 CuS、NeS 和 ExS 中央支气管的 NTCR 分别为 1.515、1.372 和 1.204。 CuS 的支气管上皮端粒明显长于非 CuS (NeS+ ExS)。当分别对女性和男性进行分析时，仅在男性中发现 CuS 和 NeS+ ExS 之间存在显着差异。肺癌病例和肺癌组织的支气管上皮的NTCR分别为1.514和1.385。结论我们的研究结果表明吸烟导致支气管上皮的端粒伸长。因此，吸烟相关癌症的致癌机制可能与许多其他癌症不同，在许多其他癌症中，由于衰老相关端粒缩短而导致的遗传不稳定性被认为发挥了作用。
BackgroundTelomeres are repetitive DNA sequences located at the ends of chromosomes. Chromosomal and genomic instability due to telomere dysfunction has been known to play an important role in the carcinogenesis of some organs.ObjectivesThe aim of this study was to examine the correlation between smoking and the telomere length of human bronchial epithelial cells in individuals with and without lung cancer.Patients and MethodsWe examined 68 non-lung cancer adult autopsy cases and 24 surgically resected cases of lung squamous cell carcinoma. Telomere lengths of the basal cells of bronchial epithelium were measured using the tissue quantitative fluorescence in situ hybridization method and were expressed in normalized telomere-to-centromere ratios (NTCRs).ResultsThe autopsied individuals included 27 current smokers (CuS), 33 never-smokers (NeS), and 8 ex-smokers (ExS). The NTCRs in the central bronchi of CuS, NeS, and ExS were 1.515, 1.372, and 1.204, respectively. The bronchial epithelial telomeres of CuS were significantly longer than those of non-CuS (NeS+ ExS). When the analysis was conducted separately for females and males, a significant difference between CuS and NeS+ ExS was recognized only for males. The NTCRs of the bronchial epithelium of lung cancer cases and lung cancer tissue are 1.514 and 1.385, respectively.ConclusionsOur findings suggest that smoking causes telomeric elongation in the bronchial epithelium. Therefore, it appears that the mechanism of carcinogenesis in smoking-related carcinomas may differ from that of many other carcinomas in which genetic instability due to aging-related telomeric shortening is assumed to play a role.