A novel initiator regulates expression of the nontissue-specific helix-loop-helix gene ME1.
A novel initiator regulates expression of the nontissue-specific helix-loop-helix gene ME1.
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一种新型启动子调节非组织特异性螺旋-环-螺旋基因 ME1 的表达。
DOI：
10.1093/nar/23.10.1696
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发表时间：
1995
影响因子：
14.9
通讯作者：
Zuber,MX
中科院分区：
文献类型：
--
作者：
Shain,DH;Neuman,T;Zuber,MX
小鼠ME1基因(Heb、Reb和GE1，在人、大鼠和鸡中分别为同源基因)是非组织特异性hellx-loop-helix(HLH)基因家族的成员，包括E2A、E2-2和Droso-phlla子代。我们研究了控制ME1基因表达的因素。ME1是一个单拷贝基因，跨越≥150kb，包含>；10个外显子。转录是由一个不寻常的启动子元件引导的，该启动子含有一个13bp的聚D(A)区，两侧是回文和反向重复序列。核糖核酸酶保护和引物延伸分析都将ME1转录起始点定位到13个碱基的多聚D(A)区的中心。ME1启动子及其近端序列是启动子活性所必需的，支持转录的基础水平，并有助于细胞类型特异性基因的表达。TATA缺失的ME1启动子利用的其他c/S元件包括一簇Sp1反应元件、E-box和一个强阻遏元件。综上所述，我们的结果表明，ME1启动子和近端启动子中的其他c/S元件在调控ME1基因的表达中起着重要作用。
The mouse ME1 gene (HEB, REB and GE1, homo-logues in human, rat and chick, respectively) Is a member of the nontissue-specific hellx-loop-helix (HLH) gene family that Includes E2A, E2-2 andDroso-phlla daughteriess. We have examined the factors that control ME1 gene expression. ME1 Is a single copy gene that spans ≥150 kb of DNA and contains >10 exons. Transcription was directed by an unusual Initiator element that contained a 13 bp poly d(A) tract flanked by palindromlc and inverted repeat sequences. Both RNase protection and primer extension analyses mapped the ME1 transcriptional start site to the center of the 13 bp poly d(A) tract. The ME1 Initiator and Its proximal sequences were required for promoter activity, supported basal levels of transcription, and contributed to cell type-specific gene expression. Other c/s-elements utilized by the TATA-less ME1 promoter Included a cluster of Sp1 response elements, E-boxes and a strong repressor. Collectively, our results suggest that the ME1 initiator and other c/s-elements In the proximal promoter play an important role In regulating ME1 gene expression.