Cell-Adhesive and Cell-Repulsive Zwitterionic Oligopeptides for Micropatterning and Rapid Electrochemical Detachment of Cells
Cell-Adhesive and Cell-Repulsive Zwitterionic Oligopeptides for Micropatterning and Rapid Electrochemical Detachment of Cells
复制标题
DOI：
10.1089/ten.tea.2011.0739
复制
发表时间：
2013-01-01
影响因子：
4.1
通讯作者：
Fukuda, Junji
中科院分区：
文献类型：
--
作者：
Kakegawa, Takahiro;Mochizuki, Naoto;Fukuda, Junji
在这项研究中，我们描述了寡肽修饰的细胞培养表面的发展，通过施加电刺激，贴壁细胞可以快速分离出来。设计了一种末端带有半胱氨酸残基的寡肽CGGGKEKEKEK，它通过金硫键与金表面结合。该多肽通过交替带电的谷氨酸(E)和赖氨酸(K)残基之间的静电力形成自组装单分子层。修饰表面致密的电中性寡肽两性离子层可抵抗蛋白质的非特异性吸附和细胞的黏附，而通过添加第二个含有RGD细胞黏附基序的寡肽(CGGGKEKEKEKGRGDSP)使其表面转变为细胞黏附。在这个金表面施加一个负的电势，裂解金-硫代键，导致寡肽层的解吸，并迅速(在2分钟内)脱离几乎所有的细胞。这种方法不仅适用于细胞膜的分离，而且还适用于将细胞微图案转移到水凝胶上。这种细胞分离的电化学方法可能是组织工程应用的有用工具。
In this study, we describe the development of oligopeptide-modified cell culture surfaces from which adherent cells can be rapidly detached by application of an electrical stimulus. An oligopeptide, CGGGKEKEKEK, was designed with a terminal cysteine residue to mediate binding to a gold surface via a gold-thiolate bond. The peptide forms a self-assembled monolayer through the electrostatic force between the sequence of alternating charged glutamic acid (E) and lysine (K) residues. The dense and electrically neutral oligopeptide zwitterionic layer of the modified surface was resistant to nonspecific adsorption of proteins and adhesion of cells, while the surface was altered to cell adhesive by the addition of a second oligopeptide (CGGGKEKEKEKGRGDSP) containing the RGD cell adhesion motif. Application of a negative electrical potential to this gold surface cleaved the gold-thiolate bond, leading to desorption of the oligopeptide layer, and rapid (within 2 min) detachment of virtually all cells. This approach was applicable not only to detachment of cell sheets but also for transfer of cell micropatterns to a hydrogel. This electrochemical approach of cell detachment may be a useful tool for tissue-engineering applications.