DISTRIBUTION OF UV LIGHT-INDUCED DAMAGE IN A DEFINED SEQUENCE OF HUMAN DNA - DETECTION OF ALKALINE-SENSITIVE LESIONS AT PYRIMIDINE NUCLEOSIDE-CYTIDINE SEQUENCES
DISTRIBUTION OF UV LIGHT-INDUCED DAMAGE IN A DEFINED SEQUENCE OF HUMAN DNA - DETECTION OF ALKALINE-SENSITIVE LESIONS AT PYRIMIDINE NUCLEOSIDE-CYTIDINE SEQUENCES
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DOI：
10.1073/pnas.78.6.3388
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
HASELTINE, WA
中科院分区：
文献类型：
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作者：
LIPPKE, JA;GORDON, LK;HASELTINE, WA
紫外光诱导损伤的分布高度重复。对人类DNA序列进行了分析。紫外光诱导的环丁烷二聚体在确定的序列中的分布是相似的，无论DNA作为完整细胞[宫颈癌HeLa细胞、CEM淋巴母细胞白血病和Tk6·β的一部分]暴露在紫外光下。淋巴细胞]。细胞环境屏蔽了核DNA，导致表观剂量减少了约50%。检测到了一种新型的紫外光损伤。用热碱处理紫外光照射的DNA，导致位于3‘’位置的胞苷到嘧啶核苷的链断裂。讨论了嘧啶核苷-胞苷损伤的化学性质和生物学意义。
The distribution of UV light-induced damage to the highly reiterated .alpha. sequence of human DNA was investigated. The distribution of UV light-induced cyclobutane dimers within a defined sequence is similar whether the DNA is exposed to UV light as part of the chromosome of intact cells [cervical carcinoma HeLa cells, CEM lymphoblastic leukemia and Tk6 .beta. lymphocytes]. The cellular environment shields the nuclear DNA, resulting in about 50% decrease in apparent dose. A new type of UV photodamage was detected. Treatment of UV light-irradiated DNA with hot alkali results in strand breaks at positions of cytidine located 3'' to pyrimidine nucleosides. The chemical nature and biological significance of the pyrimidine nucleoside-cytidine lesion is discussed.