Kinetic Pathway of 3-Helix Micelle Formation.
Kinetic Pathway of 3-Helix Micelle Formation.
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3-螺旋胶束形成的动力学途径。
DOI：
10.1021/acs.biomac.6b01831
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发表时间：
2017
影响因子：
6.2
通讯作者：
Xu,Ting
中科院分区：
文献类型：
--
作者：
Ang,JooChuan;Jung,BensonT;Dong,He;Xu,Ting
分层纳米结构自组装中一个微妙但高度相关的因素是动力学景观。分层多组分系统的自组装需要类似能量尺度上非共价相互作用的复杂平衡，这可能导致在不同时间尺度上发生多个自组装过程。我们试图了解两亲性 3 螺旋肽-PEG-脂质缀合物系统在高度稳定的 3 螺旋胶束 (3HM) 形成过程中的分层组装。 3HM 通过多个并行过程进行自组装：螺旋折叠、卷曲螺旋形成、胶束组装和烷基链堆积。我们的结果表明，3HM 形成的动力学途径主要受两个混杂因素控制：两亲物在胶束冠内形成卷曲螺旋的横向扩散和疏水性胶束核心内烷基尾部的堆积。 3HM 作为药物输送纳米载体表现出了非常理想的属性；了解各个成分在 3HM 形成动力学途径中的作用将使我们能够更好地控制动力学途径，并增强未来的设计并最终操纵动力学中间体以实现潜在的药物输送应用。
A subtle but highly pertinent factor in the self-assembly of hierarchical nanostructures is the kinetic landscape. Self-assembly of a hierarchical multicomponent system requires the intricate balance of noncovalent interactions on a similar energy scale that can result in several self-assembly processes occurring at different time scales. We seek to understand the hierarchical assemblies within an amphiphilic 3-helix peptide-PEG-lipid conjugate system in the formation process of highly stable 3-helix micelles (3HMs). 3HM self-assembles through multiple parallel processes: helix folding, coiled-coil formation, micelle assembly, and packing of alkyl chains. Our results show that the kinetic pathway of 3HM formation is mainly governed by two confounding factors: lateral diffusion of amphiphiles to form coiled-coils within the micelle corona and packing of alkyl tails within the hydrophobic micelle core. 3HM has exhibited highly desirable attributes as a drug delivery nanocarrier; understanding the role of individual components in the kinetic pathway of 3HM formation will allow us to exert better control over the kinetic pathway, as well as to enhance future design and eventually manipulate the kinetic intermediates for potential drug delivery applications.