Temporal resistance of potato tubers: Antibacterial assays and metabolite profiling of wound-healing tissue extracts from contrasting cultivars
Temporal resistance of potato tubers: Antibacterial assays and metabolite profiling of wound-healing tissue extracts from contrasting cultivars
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DOI：
10.1016/j.phytochem.2018.12.007
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发表时间：
2019-03-01
期刊：
影响因子：
3.8
通讯作者：
Stark, Ruth E.
中科院分区：
文献类型：
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作者：
Dastmalchi, Keyvan;Rodriguez, Mathiu Perez;Stark, Ruth E.
马铃薯，俗称马铃薯，是世界范围内的主食。在收获、储存和分销过程中，农作物面临机械损坏的风险。块茎皮的伤口也可能成为细菌和真菌病原体的进入点，导致巨大的农业损失。基于马铃薯块茎在伤口愈合的早期阶段，在保护性木松化周皮组织发育之前，产生代谢物来防御微生物感染的建议，我们评估了四个具有不同皮肤形态的马铃薯品种（Norkotah Russet、Atlantic、Chipeta 和 Yukon Gold）的伤口组织提取物。这些测定是在受伤后 0、1、2、3 和 7 天针对植物病原体胡萝卜软腐欧文氏菌和作为对照的非致病性大肠杆菌菌株进行的。对于每个马铃薯品种，只有极性伤口组织提取物表现出抗菌活性。早期伤口愈合时间点（第 0、1 和 2 天）的极性提取物对两种菌株的抗菌活性明显高于伤口愈合后期（第 3 和 7 天）。这些结果支持在早期时间点爆发抗菌活性。提取物的平行代谢物分析揭示了不同伤口愈合时间点化学成分的差异，并允许根据每个品种的愈合阶段识别潜在的标记化合物。通过观察各种代谢物类别随时间变化的相对数量，可以监测代谢物谱的变化，从而解释时间抵抗现象。
Solanum tuberosum, commonly known as the potato, is a worldwide food staple. During harvest, storage, and distribution the crop is at risk of mechanical damage. Wounding of the tuber skin can also become a point of entry for bacterial and fungal pathogens, resulting in substantial agricultural losses. Building on the proposal that potato tubers produce metabolites to defend against microbial infection during early stages of wound healing before protective suberized periderm tissues have developed, we assessed extracts of wound tissues from four potato cultivars with differing skin morphologies (Norkotah Russet, Atlantic, Chipeta, and Yukon Gold). These assays were conducted at 0, 1, 2, 3 and 7 days post wounding against the plant pathogen Erwinia carotovora and a non-pathogenic Escherichia coli strain that served as a control. For each of the potato cultivars, only polar wound tissue extracts demonstrated antibacterial activity. The polar extracts from earlier wound-healing time points (days 0, 1 and 2) displayed notably higher antibacterial activity against both strains than the later wound-healing stages (days 3 and 7). These results support a burst of antibacterial activity at early time points. Parallel metabolite profiling of the extracts revealed differences in chemical composition at different wound-healing time points and allowed for identification of potential marker compounds according to healing stage for each of the cultivars. It was possible to monitor the transformations in the metabolite profiles that could account for the phenomenon of temporal resistance by looking at the relative quantities of various metabolite classes as a function of time.