Rat aversion to isoflurane versus carbon dioxide
Rat aversion to isoflurane versus carbon dioxide
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DOI：
10.1098/rsbl.2012.1000
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发表时间：
2013-02-23
期刊：
影响因子：
3.3
通讯作者：
Weary, Daniel M.
中科院分区：
文献类型：
--
作者：
Wong, Devina;Makowska, I. Joanna;Weary, Daniel M.
一些专家认为，在用二氧化碳（CO2）安乐死之前用异氟烷对实验室啮齿动物进行镇静是单独用CO2安乐死的人道替代方案，但很少有研究将厌恶与这些药物进行比较。在明暗箱中对白化病大鼠进行试验，可选择留在充满异氟烷或CO2的黑暗隔室中，或逃到照明隔室中。实验1通过确认大鼠对试剂和光强度作出反应来验证该程序。在实验2中，9/16和0/16只大鼠在最初分别暴露于异氟烷和CO2时保持在暗室中，直至平躺。在实验3中，与再次暴露于异氟烷（1/16）相比，在初始（10/16）期间，更多的大鼠保持在黑暗隔室中直至横卧。这些结果表明，初始暴露于CO2比异氟烷更令人厌恶，再次暴露于异氟烷比初始暴露更令人厌恶。我们得出结论，对于先前未暴露于吸入麻醉剂的大鼠，异氟烷镇静是对单独使用CO2安乐死的改进。
Some experts suggest that sedation of laboratory rodents with isoflurane before euthanasia with carbon dioxide (CO2) is a humane alternative to euthanasia with CO2 alone, but little research has compared aversion with these agents. Albino rats were tested in a light-dark box where they had the choice between remaining in a dark compartment filling with isoflurane or CO2, or escaping to a lit compartment. Experiment 1 validated the procedure by confirming that rats responded to agent and light intensity. In experiment 2, 9/16 and 0/16 rats remained in the dark compartment until recumbent when initially exposed to isoflurane and CO2, respectively. In experiment 3, more rats remained in the dark compartment until recumbent during initial (10/16) versus re-exposure (1/16) to isoflurane. These results indicate that initial exposure to CO2 is more aversive than isoflurane, and that re-exposure to isoflurane is more aversive than initial exposure. We conclude that sedation with isoflurane is a refinement over euthanasia with CO2 alone for rats that have not been previously exposed to inhalant anaesthetics.