Seroprevalence of tularemia in wild bears and hares in Japan
Seroprevalence of tularemia in wild bears and hares in Japan
复制标题
日本野熊和野兔中兔热病的血清流行率
DOI：
10.1111/j.1863-2378.2011.01422.x
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
et. Al
中科院分区：
文献类型：
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作者：
Hotta A;Tanabayashi K;Yamamoto Y;Fujita O;Uda A;Mizoguchi T;et. Al
土拉菌病是由土拉弗朗西斯菌引起的人畜共患传染病。该病原体在日本的分布尚未得到很好的研究。在这项研究中，在日本野生黑熊和野兔兔热病的血清阳性率进行了测定。在1998年至2009年期间，对431只日本黑熊（Ursus thibetanus）和293只日本野兔（Lepus brachurus）的血液样本进行了抗F抗体检测。土拉菌病通过微量凝集试验（MA）或酶联免疫吸附试验。经免疫印迹法（WB）和间接免疫荧光法（IFA）确证试验，8份日本黑熊血清均为阳性。这八只熊都是日本本岛东北部的居民，那里曾报告过人类兔热病。这些结果表明，日本黑熊可以作为兔热病监测的哨点，并有助于了解F.土拉菌遍布全国。这是首次报道抗F抗体的检测。日本黑熊中的土拉热。
Tularemia is a zoonotic disease caused byFrancisella tularensis. The distribution of the pathogen in Japan has not been studied well. In this study, seroprevalence of tularemia among wild black bears and hares in Japan was determined. Blood samples collected from 431 Japanese black bears (Ursus thibetanus japonicus) and 293 Japanese hares (Lepus brachurus) between 1998 and 2009 were examined for antibodies againstF. tularensisby micro‐agglutination test (MA) or enzyme‐linked immunosorbent assay. By subsequent confirmatory tests using western blot (WB) and indirect immunofluorescence assay (IFA), eight sera from Japanese black bears were definitely shown to be seropositive. All of these eight bears were residents of the northeastern part of main‐island of Japan, where human tularemia had been reported. On the other hand, no seropositive Japanese hares were found. These results suggest that Japanese black bears can serve as sentinel for tularemia surveillance and may help understand the distribution ofF. tularensisthroughout the country. This is the first report on detection of antibody toF. tularensisin black bears of Japan.