Gonadal structure of the serial‐sex changing gobiid fish Trimma okinawae
Gonadal structure of the serial‐sex changing gobiid fish Trimma okinawae
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DOI：
10.1111/j.1440-169x.2004.00774.x
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发表时间：
2005-01
期刊：
影响因子：
4.6
通讯作者：
Yasuhisa Kobayashi;T. Sunobe;Tohru Kobayashi;Y. Nagahama;Masaru Nakamura
中科院分区：
文献类型：
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作者：
Yasuhisa Kobayashi;T. Sunobe;Tohru Kobayashi;Y. Nagahama;Masaru Nakamura
为了获得内源性激素在连续变性戈壁鱼Trimma okinawae性别变化中所起的作用的基本信息，对雄性和雌性阶段的性腺结构进行组织学观察。在雌性期和雄性期鱼的卵巢和睾丸中通过超微结构观察到产生类固醇的细胞（SPC；睾丸中的间质细胞）。此外，还通过免疫组织化学方法检查了 P450 胆固醇侧链裂解 (scc) 的性腺表达。观察到雌性和雄性阶段的鱼的性腺同时具有卵巢和睾丸。雌性期鱼已成熟，具有许多发育的卵黄发生卵母细胞，而雄性期个体的卵巢未成熟，具有许多处于核周阶段的卵黄发生前卵母细胞。处于不同性阶段的鱼的睾丸都有活跃的生精生殖细胞。雌性期鱼卵巢中的 SPC 细胞器具有活跃的类固醇生成结构。相比之下，雄性期鱼卵巢中的 SPC 没有显示出类固醇产生的活性结构。雌性鱼卵巢中针对scc抗体的免疫反应非常强，而雄性鱼卵巢中的免疫反应非常弱。在睾丸中，从双相男性/女性获得了中等免疫阳性信号。
In order to obtain basic information about the role played by endogenous sex hormones in bringing about sex changes in the serial‐sex changing gobiid fish Trimma okinawae, the gonadal structure of male and female phases were observed histologically. Steroid‐producing cells (SPC; Leydig cells in a testis) were observed ultrastructurally in the ovaries and testes of both female‐phase and male‐phase fish. In addition, gonadal expression of P450 cholesterol side‐chain‐cleavage (scc) was examined immunohistochemically. Gonads of fish in female and male phases were observed to have both ovaries and testes simultaneously. Female‐phase fish had matured with many developed vitellogenic oocytes, while male‐phase individuals had immature ovaries with many numbers of previtellogenic oocytes at the perinucleolus stage. Testes of fish in different sexual phases had active spermatogenic germ cells. Organellae of SPC in the ovaries of female‐phase fish had active structures of steroid production. In contrast, SPC in the ovaries of male‐phase fish did not show active structures of steroid production. Immunopositive reactions against the scc antibody in the ovaries of female‐phase fish were very strong, but immunoreactions in the ovaries of male‐phase fish were very weak. In the testis, moderate immunopositive signals were obtained from dual‐phase male/females.