Chlorophyll Fluorescence Imaging of the Chlorophyll Fluorescence Induction Phenomenon for Plant Health Monitoring
Chlorophyll Fluorescence Imaging of the Chlorophyll Fluorescence Induction Phenomenon for Plant Health Monitoring
复制标题
用于植物健康监测的叶绿素荧光诱导现象的叶绿素荧光成像
DOI：
10.2525/ecb.47.101
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
H. Nishina
中科院分区：
文献类型：
--
作者：
K. Takayama;H. Nishina
叶绿素荧光成像作为一种无损评价植物光合功能的方法是有用的。叶绿素荧光强度的动态变化，被称为叶绿素荧光诱导现象，可以观察到用稳定强度的激发光照射黑暗适应的绿叶。在此过程中，叶绿素荧光强度的时间过程以诱导曲线的形式呈现，随植物的健康状况而变化。叶绿素荧光诱导现象的成像可用于监测植物的健康状况。本文介绍了叶绿素荧光成像系统在温室番茄作物健康监测中的研究进展。我们首先开发了成像系统的原型，以确认在单叶和整株植物中检测人工诱导光胁迫的性能。在成功检测光胁迫引起的光合功能障碍的基础上，我们将叶绿素荧光成像系统应用于半商业化温室番茄作物叶绿素荧光诱导曲线的测量。通过对温室内南北通道种植的20株番茄植株的诱导曲线进行比较，发现两株植株具有独特的诱导曲线模式。其中一株在测量7天后出现了明显的生理功能障碍症状。因此，我们的叶绿素荧光成像系统似乎是一个有用的工具，植物健康监测园艺生产。
Chlorophyll fluorescence imaging is useful as a non-destructive method for evaluating photosynthetic function of plants. A dynamic change in chlorophyll fluorescence intensity, known as the chlorophyll fluorescence induction phenomenon, can be observed by illuminating a dark-adapted green leaf with a stable intensity excitation light. The time course of the chlorophyll fluorescence intensity during this phenomenon, presented as an induction curve, varies depending on the health of the plant. Imaging of the chlorophyll fluorescence induction phenomenon may be used to monitor the health status of plants. In this review, we introduce studies on our chlorophyll fluorescence imaging system for plant health monitoring of tomato crops grown in a greenhouse. We first developed a prototype of the imaging system to confirm performance on detection of artificially induced light stress in a single leaf and whole plant. Based on the successful detection of photosynthetic dysfunction caused by light stress using the prototype, we applied our chlorophyll fluorescence imaging system to measurements of the chlorophyll fluorescence induction curves of tomato crops grown in a semi-commercial greenhouse. Upon comparing the induction curves of 20 tomato crops planted on a north-south lane in the greenhouse, we found two plants with unique induction curve patterns. One of these two plants showed visible symptoms of physiological dysfunction 7 days after measurement. Thus, our chlorophyll fluorescence imaging system appears to be a useful tool for plant health monitoring in horticultural production.