Differential induction of heat shock mRNA in oligodendrocytes, microglia, and astrocytes following hyperthermia
Differential induction of heat shock mRNA in oligodendrocytes, microglia, and astrocytes following hyperthermia
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DOI：
10.1016/s0169-328x(96)00138-6
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发表时间：
1997-05-01
期刊：
影响因子：
--
通讯作者：
Brown, IR
中科院分区：
文献类型：
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作者：
Foster, JA;Brown, IR
在兔前脑中进行响应于生理相关的体温增加2.6摄氏度的热休克蛋白70 mRNA诱导的时程分析。一个协议，结合原位杂交和细胞化学在同一组织切片，以确定反应性胶质细胞类型。星形胶质细胞，小胶质细胞和少突胶质细胞的细胞化学标记物与地高辛标记的热休克蛋白70核糖核酸探针，允许在高分辨率的mRNA定位相结合。本实验观察了兔前脑皮质第一层、海马裂、胼胝体和海马伞四个胶质细胞体富集区。最大的热休克蛋白70 mRNA的诱导观察在2和3小时的高温动物。共定位分析表明hsp70 mRNA在少突胶质细胞和小胶质细胞中被诱导表达，而在前脑GFAP阳性星形胶质细胞中不表达。此外，进行细胞计数，这表明几乎所有的少突胶质细胞诱导hsp70 mRNA，而小胶质细胞的亚群作出反应。这些数据是一致的概念，少突胶质细胞，小胶质细胞和星形胶质细胞表现出不同的阈值激活的热休克反应后，生理相关的体温升高。
A time course analysis of hsp70 mRNA induction in response to a physiologically relevant increase in body temperature of 2.6 degrees C was performed in the rabbit forebrain. A protocol that combined in situ hybridization and cytochemistry on the same tissue section was employed to identify reactive glial cell types. Cytochemical markers for astrocytes, microglia, and oligodendrocytes were utilized in combination with a DIG-labelled hsp70 riboprobe, which permitted mRNA localization at high resolution. Four glial cell body-enriched regions of the rabbit forebrain were examined, namely, cortical layer 1, hippocampal fissure, corpus callosum, and fimbria. Maximal hsp70 mRNA induction was observed in 2 and 3 h hyperthermic animals. The colocalization analysis demonstrated that hsp70 mRNA was induced in oligodendrocytes and microglia, but not in forebrain GFAP positive astrocytes. In addition, cell counts were performed which showed that almost all oligodendrocytes induced hsp70 mRNA while a subpopulation of microglial cells responded. These data are consistent with the notion that oligodendrocytes, microglia, and astrocytes exhibit distinct thresholds for activation of the heat shock response following a physiologically relevant increase in body temperature.