Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients.
Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients.
复制标题
DOI：
10.1155/2015/281870
复制
发表时间：
2015
影响因子：
--
通讯作者：
Aragona P
中科院分区：
文献类型：
--
作者：
Dobrowolski D;Orzechowska-Wylegala B;Wowra B;Wroblewska-Czajka E;Grolik M;Szczubialka K;Nowakowska M;Puzzolo D;Wylegala EA;Micali A;Aragona P
目的.培养口腔黏膜上皮移植术治疗无虹膜角膜上皮缺损的疗效观察方法.研究对象为无虹膜患者（13例患者; 17只眼），伴有累及视轴的不规则血管性结膜血管翳，接受了培养上皮的自体移植。对于该程序，口腔粘膜上皮细胞从颊粘膜获得，并进行进一步的酶处理。将单细胞悬浮液接种在预先制备的裸露羊膜上。在存在用丝裂霉素C灭活的3 T3成纤维细胞单层的情况下，在培养皿插入物上进行培养。培养物进行7天。将口腔粘膜上皮层及其羊膜载体移植于无虹膜患者角膜缘干细胞完全或次全缺乏的手术剥脱的角膜表面。结果衡量标准。评价角膜表面、上皮规则性和视力改善情况。结果在观察期结束时，76.4%的眼睛具有规则的透明上皮，23.5%出现上皮缺损或中央角膜混浊; 88.2%的病例视力增加。VA范围从术前HM 0.05到术后HM高达0.1。结论应用培养的口腔粘膜上皮恢复了角膜表面的规则上皮，并适度改善了视觉质量。
Purpose. Efficacy of cultivated oral mucosa epithelial transplantation (COMET) procedure in corneal epithelium restoration of aniridia patients. Methods. Study subjects were aniridia patients (13 patients; 17 eyes) with irregular, vascular conjunctival pannus involving visual axis who underwent autologous transplantation of cultivated epithelium. For the procedure oral mucosa epithelial cells were obtained from buccal mucosa with further enzymatic treatment. Suspension of single cells was seeded on previously prepared denuded amniotic membrane. Cultures were carried on culture dishes inserts in the presence of the inactivated with Mitomycin C monolayer of 3T3 fibroblasts. Cultures were carried for seven days. Stratified oral mucosa epithelium with its amniotic membrane carrier was transplanted on the surgically denuded corneal surface of aniridia patients with total or subtotal limbal stem cell deficiency. Outcome Measures. Corneal surface, epithelial regularity, and visual acuity improvement were evaluated. Results. At the end of the observation period, 76.4% of the eyes had regular transparent epithelium and 23.5% had developed epithelial defects or central corneal haze; in 88.2% of cases visual acuity had increased. VA range was from HM 0.05 before the surgery to HM up to 0.1 after surgery. Conclusion. Application of cultivated oral mucosa epithelium restores regular epithelium on the corneal surface with moderate improvement in quality of vision.