Optimization for extracellular polymeric substances extraction of microbial aggregates.
Optimization for extracellular polymeric substances extraction of microbial aggregates.
复制标题
DOI：
10.2166/wst.2015.043
复制
发表时间：
2015-04
期刊：
影响因子：
--
通讯作者：
Liang Zhu;Hai-tian Yu;Yimei Liu;Han-ying Qi;Xiang-yang Xu
中科院分区：
文献类型：
--
作者：
Liang Zhu;Hai-tian Yu;Yimei Liu;Han-ying Qi;Xiang-yang Xu
胞外聚合物质（EPS）是微生物聚集体中重要的大分子成分。研究了超声+阳离子交换树脂(CER) +硫化物、超声+甲酰胺+ NaOH、超声+热三种EPS提取方法，并利用化学分析和三维激发发射矩阵（3D-EEM）比较了松散絮凝体和致密好氧颗粒中提取EPS的组分差异。结果表明：超声+甲酰胺+氢氧化钠法和超声+热法均能有效提取EPS，超声+ CER +硫化物法不能提取污泥样品中的多糖（PS）和蛋白质（PN）。3D-EEM分析表明，B/D峰、C/E/F峰和A/G峰属于pn样、腐植酸样和黄腐酸样荧光团。通过超声+热法提取的EPS可以检测到所有的荧光团。希望这将为微生物聚集体的EPS相互作用机制提供更多的信息。
The extracellular polymeric substances (EPS) are important macromolecular components in microbial aggregates. The three EPS extraction methods - ultrasound + cation exchange resins (CER) + sulfide, ultrasound + formamide + NaOH, and ultrasound + heat - were investigated in the study, and the component differences of extracted EPS from the loose flocs and dense aerobic granules were compared using chemical analysis and three-dimensional excitation-emission matrix (3D-EEM). Results showed that the contents of EPS were extracted effectively by ultrasound + formamide + NaOH and ultrasound + heat methods, and the ultrasound + CER + sulfide method did not extract the polysaccharides (PS) or protein (PN) contents from the sludge samples. The 3D-EEM analysis indicated that the nature of peak B/D, peak C/E/F, and peak A/G were attributed to PN-like, humic acid-like and fulvic acid-like fluorophores. All fluorophores can be detected from the EPS extracted through the ultrasound + heat method. Hopefully this will provide more information about the EPS interaction mechanism of microbial aggregates.