C2C12 myotubes inhibit the proliferation and differentiation of 3T3-L1 preadipocytes by reducing the expression of glucocorticoid receptor gene
C2C12 myotubes inhibit the proliferation and differentiation of 3T3-L1 preadipocytes by reducing the expression of glucocorticoid receptor gene
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DOI：
10.1016/j.bbrc.2016.02.063
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发表时间：
2016-03-25
影响因子：
3.1
通讯作者：
Chen, Jie
中科院分区：
文献类型：
--
作者：
Chu, Weiwei;Wei, Wei;Chen, Jie
肥胖与胰岛素抵抗、糖尿病和代谢综合征有关，是公认的健康危险因素。肌细胞-脂肪细胞串扰模型在研究肌肉和脂肪发育的相互作用中具有重要意义。以往的相关研究主要集中在脂肪细胞对肌细胞活性的影响，而肌管对前脂肪细胞发育的影响尚不清楚。本研究就是为了解决这一问题而进行的。首先，共培养实验表明，分化的C2 C12肌管可抑制3 T3-L1前脂肪细胞的增殖、细胞周期和分化，并诱导其凋亡。与C2 C12肌管共培养后，3 T3-L1前脂肪细胞对糖皮质激素（glucocorticoids，GCs）的敏感性降低。此外，C2 C12肌管抑制了3 T3-L1前脂肪细胞糖皮质激素受体（GR）的mRNA和蛋白表达，提示了GCs敏感性降低的可能机制。综上所述，我们得出结论，C2 C12肌管抑制3 T3-L1前脂肪细胞的增殖和分化，通过减少GR的表达。这些数据表明，减少GR的管理肌因子可能是一个有前途的治疗肥胖症或糖尿病患者。(C)2016 Elsevier Inc. All rights reserved.
Obesity is a well-established risk factor to health for its relationship with insulin resistance, diabetes and metabolic syndrome. Myocyte-adipocyte crosstalk model plays a significant role in studying the interaction of muscle and adipose development. Previous related studies mainly focus on the effects of adipocytes on the myocytes activity, however, the influence of myotubes on the preadipocytes development remains unclear. The present study was carried out to settle this issue. Firstly, the co-culture experiment showed that the proliferation, cell cycle, and differentiation of 3T3-L1 preadipocytes were arrested, and the apoptosis was induced, by differentiated C2C12 myotubes. Next, the sensitivity of 3T3-L1 preadipocytes to glucocorticoids (GCs), which was well known as cell proliferation, differentiation, apoptosis factor, was decreased after co-cultured with C2C12 myotubes. What's more, our results showed that C2C12 myotubes suppressed the mRNA and protein expression of glucocorticoid receptor (GR) in 3T3-L1 preadipocytes, indicating the potential mechanism of GCs sensitivity reduction. Taken together, we conclude that C2C12 myotubes inhibited 3T3-L1 preadipocytes proliferation and differentiation by reducing the expression of GR. These data suggest that decreasing GR by administration of myokines may be a promising therapy for treating patients with obesity or diabetes. (C) 2016 Elsevier Inc. All rights reserved.