CONTROL OF COLE1 PLASMID REPLICATION - BINDING OF RNA-I TO RNA-II AND INHIBITION OF PRIMER FORMATION
CONTROL OF COLE1 PLASMID REPLICATION - BINDING OF RNA-I TO RNA-II AND INHIBITION OF PRIMER FORMATION
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DOI：
10.1016/0092-8674(86)90369-7
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发表时间：
1986-10-10
期刊：
影响因子：
64.5
通讯作者：
TOMIZAWA, J
中科院分区：
文献类型：
--
作者：
TOMIZAWA, J
ColE1 DNA复制的引物RNA II的形成受反义转录物RNA I的调控。将长度为100-360个核苷酸的RNA II暴露于RNA I可抑制后续引物的形成。然而，引物通常形成于长度超过360个核苷酸的转录本。因此，RNA I与RNA II的结合速率对调控至关重要。不同长度的RNA II转录本的结合率不同。长度超过200个核苷酸的转录本比短的转录本结合得更快。Rom蛋白增强RNA I与这些较长转录本的结合，而抑制与较短转录本的结合。长度超过360个核苷酸的转录本对RNA I不敏感不是由于RNA I不结合，而是由于这种结合对引物的形成没有影响。
Formation of the primer for ColE1 DNA replication from the primary transcript, RNA II, is regulated by an antisense transcript, RNA I. Exposure of elongating RNA II 100-360 nucleotides long to RNA I inhibits subsequent primer formation. However, primer forms normally for transcripts longer than 360 nucleotides. Therefore, the rate of binding of RNA I to RNA II is crucial to regulation. The binding rate varies among RNA II transcripts of different lengths. Transcripts longer than 200 nucleotides are bound faster than shorter ones. The Rom protein enhances binding of RNA I to these longer transcripts, whereas it suppresses binding to some shorter ones. The insensitivity to RNA I of transcripts longer than 360 nucleotides is not due to the absence of RNA I binding but to this binding having no effect on primer formation.