Contribution of phosphatidylserine to Rho1- and Pkc1-related repolarization of the actin cytoskeleton under stressed conditions in Saccharomyces cerevisiae
Contribution of phosphatidylserine to Rho1- and Pkc1-related repolarization of the actin cytoskeleton under stressed conditions in Saccharomyces cerevisiae
复制标题
酿酒酵母应激条件下磷脂酰丝氨酸对肌动蛋白细胞骨架 Rho1 和 Pkc1 相关复极化的贡献
DOI：
10.1080/21541248.2017.1339766
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Inoue Yoshiharu
中科院分区：
文献类型：
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作者：
Nomura Wataru;Inoue Yoshiharu
芽殖酵母（Saccharomyces）经历极化细胞生长，这与肌动蛋白极化有关。Rho 1是酿酒酵母中的Rho型GTP酶之一，通过与该酵母中唯一的蛋白激酶C Pkc 1相互作用，对维持细胞极化生长和肌动蛋白极化以及控制下游信号通路Pkc 1-Mpk 1 MAP激酶级联起关键作用。Pkc 1-Mpk 1 MAP激酶级联反应对热休克应激条件下肌动蛋白的复极化非常重要。我们最近报道，磷脂酰丝氨酸（PS），膜磷脂成分，在Rho 1和Pkc 1之间的物理相互作用以及Pkc 1-Mpk 1 MAP激酶级联的激活中发挥了关键作用。然而，目前尚不清楚PS是否通过调节Rho 1和Pkc 1之间的物理相互作用参与肌动蛋白极化。我们在此证明，C1结构域的Pkc 1，这是负责与Rho 1的相互作用，是至关重要的Rho 1调节肌动蛋白极化。我们还发现，肌动蛋白复极化在热休克应激条件下受损的突变缺陷inCHO 1编码PS合酶。这些结果表明，PS有助于肌动蛋白的极化，其中Rho 1和Pkc 1起着至关重要的作用。
The budding yeastSaccharomyces cerevisiaeundergoes polarized cell growth, which is established in association with actin polarization. Rho1, one of the Rho-type GTPases inS. cerevisiae, is crucial for maintaining polarized cell growth and actin polarization and controlling the downstream signaling pathway, the Pkc1-Mpk1 MAP kinase cascade, through a physical interaction with Pkc1, the sole protein kinase C in this yeast. The Pkc1-Mpk1 MAP kinase cascade is important for the repolarization of actin under heat shock-stressed conditions. We recently reported that phosphatidylserine (PS), a membrane phospholipid component, played a pivotal role in the physical interaction between Rho1 and Pkc1 as well as the activation of the Pkc1-Mpk1 MAP kinase cascade. However, it currently remains unclear whether PS is involved in actin polarization by regulating the physical interaction between Rho1 and Pkc1. We herein demonstrated that the C1 domain of Pkc1, which is responsible for the interaction with Rho1, was crucial for Rho1-regulated actin polarization. We also found that actin repolarization under heat shock-stressed conditions was impaired in a mutant defective inCHO1encoding PS synthase. These results suggest that PS contributes to actin polarization in which Rho1 and Pkc1 play a crucial role.