HUMAN HEPATOCELLULAR-CARCINOMA CELL-LINES SECRETE THE MAJOR PLASMA-PROTEINS AND HEPATITIS-B SURFACE-ANTIGEN
HUMAN HEPATOCELLULAR-CARCINOMA CELL-LINES SECRETE THE MAJOR PLASMA-PROTEINS AND HEPATITIS-B SURFACE-ANTIGEN
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DOI：
10.1126/science.6248960
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发表时间：
1980-01-01
期刊：
影响因子：
56.9
通讯作者：
ADEN, DP
中科院分区：
文献类型：
--
作者：
KNOWLES, BB;HOWE, CC;ADEN, DP
对两种新的人肝癌细胞系的细胞培养液的分析表明，17 种主要的人血浆蛋白是由这些细胞合成和分泌的。其中一种细胞系 Hep 3B 也产生乙型肝炎病毒表面抗原的两种主要多肽。当 Hep 3B 注射到无胸腺小鼠体内时，会出现转移性肝细胞癌。这些细胞系为研究血浆蛋白生物合成以及乙型肝炎病毒基因组与致瘤性的关系提供了实验模型。
Analysis of the cell culture fluid from two new human hepatoma-derived cell lines reveals that 17 of the major human plasma proteins are synthesized and secreted by these cells. One of these cell lines, Hep 3B, also produces the two major polypeptides of the hepatitis B virus surface antigen. When Hep 3B is injected into athymic mice, metastatic hepatocellular carcinomas appear. These cell lines provide experimental models for investigation of plasma protein biosynthesis and the relation of the hepatitis B virus genome to tumorigenicity.