Evaluation of hydrogel matrices for vessel bioplotting: Vascular cell growth and viability.
Evaluation of hydrogel matrices for vessel bioplotting: Vascular cell growth and viability.
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DOI：
10.1002/jbm.a.35590
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发表时间：
2016-03
期刊：
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通讯作者：
R. Singh;Bapi Sarker;Raquel Silva;R. Detsch;B. Dietel;C. Alexiou;A. Boccaccini;I. Cicha
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文献类型：
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作者：
R. Singh;Bapi Sarker;Raquel Silva;R. Detsch;B. Dietel;C. Alexiou;A. Boccaccini;I. Cicha
开发与血管细胞相容的基质是组织工程中最具挑战性的任务之一。在这里，我们比较了血管细胞在不同水凝胶上的生长，作为血管组织生物绘图的潜在材料。制备含有与1%w/v的蛋白质溶液（丝纤蛋白、明胶、角蛋白或弹性蛋白）共混的藻酸盐溶液（Alg，2%，w/v）的制剂。将人脐静脉内皮细胞（EC）、平滑肌细胞（SMC）和成纤维细胞在水凝胶上培养7天。在第3天和第7天使用荧光染色观察细胞数量和形态。采用WST法分析细胞代谢活性。与纯Alg相比，Alg/角蛋白、Alg/明胶和Alg/丝素蛋白为EC提供了极好的表面，支持其附着、生长、铺展和代谢活动。SMC在Alg/丝素蛋白和Alg/角蛋白水凝胶上表现出最好的定植和生长，而在含有弹性蛋白的水凝胶上观察到细胞聚集。成纤维细胞的生长增强Alg/弹性蛋白，并强烈改善丝素蛋白和角蛋白含有水凝胶。与先前使用藻酸双酯钠-明胶交联凝胶的研究相反，Alg/明胶共混物水凝胶为成纤维细胞提供了不太有利的支架。总之，最有希望的结果是用含有丝素蛋白和角蛋白的水凝胶获得的，该水凝胶支持所有类型的血管细胞的生长。© 2015威利期刊公司. J Biomed Mater Res Part A：104A：577-585，2016.
Developing matrices biocompatible with vascular cells is one of the most challenging tasks in tissue engineering. Here, we compared the growth of vascular cells on different hydrogels as potential materials for bioplotting of vascular tissue. Formulations containing alginate solution (Alg, 2%, w/v) blended with protein solutions (silk fibroin, gelatin, keratin, or elastin) at 1% w/v were prepared. Human umbilical vein endothelial cells (ECs), smooth muscle cells (SMCs), and fibroblasts were cultivated on hydrogels for 7 days. Cell number and morphology was visualised using fluorescent staining at day 3 and 7. Cell metabolic activity was analysed using WST assay. Compared to pure Alg, Alg/keratin, Alg/gelatin and Alg/silk fibroin provided superb surfaces for ECs, supporting their attachment, growth, spreading and metabolic activity. SMCs showed best colonization and growth on Alg/silk fibroin and Alg/keratin hydrogels, whereas on elastin-containing hydrogels, cell clustering was observed. Fibroblasts growth was enhanced on Alg/elastin, and strongly improved on silk fibroin- and keratin-containing hydrogels. In contrast to the previous studies with alginate dialdehyde-gelatin crosslinked gels, Alg/gelatin blend hydrogels provided a less favourable scaffold for fibroblasts. Taken together, the most promising results were obtained with silk fibroin- and keratin-containing hydrogels, which supported the growth of all types of vascular cells. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 577-585, 2016.