Characterization of a laboratory-generated variant of BPS β-lactamase from Burkholderia pseudomallei that hydrolyses ceftazidime
Characterization of a laboratory-generated variant of BPS β-lactamase from Burkholderia pseudomallei that hydrolyses ceftazidime
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DOI：
10.1093/jac/dkf208
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发表时间：
2002-11-01
影响因子：
5.2
通讯作者：
Yuen, KY
中科院分区：
文献类型：
--
作者：
Ho, PL;Cheung, TKM;Yuen, KY
Burkholderia pseudomallei produces an Ambler class A beta-lactamase, known as BPS-1. The beta-lactamase gene from a laboratory-derived, ceftazidime-resistant strain of B. pseudomallei (LH-1-2) was cloned and expressed in Escherichia coli. The beta-lactamase, named BPS-1m, had an identical isoelectric focusing point (pI 7.7) to that of BPS-1, but differed in having a stronger hydrolytic activity against ceftazidime. Susceptibility testing showed that BPS-1m when expressed in E. coli conferred resistance to ceftazidime (MIC greater than or equal to 32 mg/L). The amino acid sequence of BPS-1m differed from that of BPS-1 by a Pro-to-Ser change at position 167 in the omega loop.
