Optical imaging of bacterial infection in living mice using a fluorescent near-infrared molecular probe
Optical imaging of bacterial infection in living mice using a fluorescent near-infrared molecular probe
复制标题
DOI：
10.1021/ja0665592
复制
发表时间：
2006-12-27
影响因子：
15
通讯作者：
Smith, Bradley D.
中科院分区：
文献类型：
--
作者：
Leevy, W. Matthew;Gammon, Seth T.;Smith, Bradley D.
通过将近红外碳菁荧光团连接到含有两个锌（II）吡啶二甲基胺（Zn-DPA）单元的亲和基团上，合成了一种光学成像探针。该探针对细菌表面具有很强的选择性亲和力，并用于革兰氏阳性菌感染的成像。金黄色和革兰氏阴性E.活裸鼠中的大肠杆菌。静脉注射后，探针选择性地聚集在小鼠大腿肌肉中的局部细菌感染部位。
An optical imaging probe was synthesized by attaching a near-infrared carbocyanine fluorophore to an affinity group containing two zinc(II) dipicolylamine (Zn-DPA) units. The probe has a strong and selective affinity for the surfaces of bacteria, and it was used to image infections of Gram-positiveS. aureusand Gram-negativeE. colibacteria in living nude mice. After intravenous injection, the probe selectively accumulates at the sites of localized bacterial infections in the thigh muscles of the mice.