CONSTANS mediates between the circadian clock and the control of flowering in Arabidopsis
CONSTANS mediates between the circadian clock and the control of flowering in Arabidopsis
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DOI：
10.1038/35074138
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发表时间：
2001-04-26
期刊：
影响因子：
64.8
通讯作者：
Coupland, G
中科院分区：
文献类型：
--
作者：
Suárez-López, P;Wheatley, K;Coupland, G
开花通常是由植物暴露在适当的日照长度引发的。这种反应需要一个称为昼夜节律钟的内源性计时器来测量白天或黑夜的持续时间（1）。这个计时器还控制着基因表达和行为模式（如叶片运动）的每日节奏。几个拟南芥突变影响昼夜节律过程和开花时间（2-10）;但这些突变对昼夜节律钟的影响如何与它们对开花的影响相关仍然未知。在这里，我们表明CONSTANS（CO）的表达，一种响应长日照加速开花的基因（11），受到生物钟和日照长度的调节。一氧化碳靶基因的表达，称为FLOWERLOCUS T（FT），被限制在一天中的一个类似的时间作为CO的表达。三个突变，影响昼夜节律和开花时间改变CO和FT的表达方式是一致的，其对开花的影响。此外，这种突变体的晚开花表型是通过过表达CO来纠正的。因此，CO在生物钟和开花控制之间起作用，这表明日长调节开花时间的机制。
Flowering is often triggered by exposing plants to appropriate day lengths. This response requires an endogenous timer called the circadian clock to measure the duration of the day or night(1). This timer also controls daily rhythms in gene expression and behavioural patterns such as leaf movements. Several Arabidopsis mutations affect both circadian processes and flowering time(2-10); but how the effect of these mutations on the circadian clock is related to their influence on flowering remains unknown. Here we show that expression of CONSTANS (CO), a gene that accelerates flowering in response to long days(11), is modulated by the circadian clock and day length. Expression of a CO target gene, called FLOWERING LOCUS T (FT), is restricted to a similar time of day as expression of CO. Three mutations that affect circadian rhythms and flowering time alter CO and FT expression in ways that are consistent with their effects on flowering. In addition, the late flowering phenotype of such mutants is corrected by overexpressing CO. Thus, CO acts between the circadian clock and the control of flowering, suggesting mechanisms by which day length regulates flowering time.