Determination of cabergoline and L-dopa in human plasma using liquid chromatography-tandem mass spectrometry
Determination of cabergoline and L-dopa in human plasma using liquid chromatography-tandem mass spectrometry
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DOI：
10.1016/s1570-0232(03)00279-4
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发表时间：
2003-07-15
影响因子：
3
通讯作者：
Sakoda, S
中科院分区：
文献类型：
--
作者：
Igarashi, K;Hotta, K;Sakoda, S
采用LC-MS-MS法测定人血浆中卡麦角林和L-多巴的浓度。采用反相液相色谱直接测定有机溶剂或酸脱蛋白血浆样品。采用电喷雾电离LC-MS-MS多反应监测方法，测定人血浆中卡麦角林和L-多巴的m/z381和m/z198。卡麦角林和L多巴的线性范围分别为5~250pg/ml和1~200 ng/ml。卡麦角林的检出限约为2pg/ml，L多巴的检出限约为0.1pg/ml。方法用于卡麦角林和L-多巴的血药浓度测定。精密度：卡麦角林浓度为13.8~26.2pg/ml，变异系数为3.8%~10.5%；L多巴浓度为302.5~522.1 ng/ml，变异系数为2.9%~8.9%。结果表明，该方法非常适合于常规分析。(C)2003 Elsevier Science B.V.保留所有权利。
We determined cabergoline and L-dopa in human plasma using liquid chromatography-mass spectrometry with tandem mass spectrometry (LC-MS-MS). The deproteinized plasma samples with organic solvent or acid were analyzed directly by reversed-phase liquid chromatography. Using multiple reaction monitoring (MRM, product ions m/z 381 of m/z 452 for cabergoline and m/z 152 of m/z 198 for L-dopa) on LC-MS-MS with electrospray ionization (ESI), cabergoline and L-dopa in human plasma were determined. Calibration curves of the method showed a good linearity in the range 5-250 pg/ml for cabergoline and 1-200 ng/ml for L-dopa, respectively. The limit of determination was estimated to be approximately 2 pg/ml for cabergoline and approximately 0.1 ng/ml for L-dopa, respectively. The method was applied to the analysis of cabergoline and L-dopa in plasma samples from patients treated with these drugs. The precision of analysis showed coefficients of variation ranging from 3.8% to 10.5% at cabergoline concentration of 13.8-26.2 pg/ml and from 2.9% to 8.9% at an L-dopa concentration of 302.5-522.1 ng/ml in patient plasma. As a result, the procedure proved to be very suitable for routine analysis. (C) 2003 Elsevier Science B.V. All rights reserved.