Oral Microflora: A Comparative Study in HIV and Normal Patients
Oral Microflora: A Comparative Study in HIV and Normal Patients
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DOI：
10.1007/s12070-011-0370-z
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发表时间：
2014-01-01
影响因子：
0.6
通讯作者：
Sreedharan, Suja
中科院分区：
文献类型：
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作者：
Hegde, Mahesh Chandra;Kumar, Abhijit;Sreedharan, Suja
该研究旨在比较正常人和艾滋病毒感染者的口腔微生物群。该研究试图确定艾滋病毒感染者口腔微生物群的显着变化。进行抗生素敏感性测试以确定抗生素耐药性的增加。这是一项在三级护理中心进行的为期两年半的前瞻性研究。研究小组由 80 名受试者组成，分为对照组和艾滋病毒两组。该群体的年龄范围为 9-75 岁。该组的平均年龄为 39.7 岁。男女比例为2.75:1。结核病是艾滋病毒感染者中最常见的机会性感染。最常见的共生微生物是草绿色链球菌，占 60%，其次是肺炎链球菌，占 23.33%。 HIV组：最常见的共生微生物是草绿色链球菌，占42%；其次是微球菌属。 22% 的情况下。 6% 的病例中分离出肺炎链球菌。对照组中 55.56% 的病例中草绿色链球菌菌落计数大幅增长，而 HIV 组中的这一数字为 62.5%。微球菌属从 HIV 组的 11 名受试者中分离出来，但没有从对照组中分离出来。 HIV 组中 50% 的受试者显示克雷伯氏菌大量生长。而对照组仅显示出适度且微弱的增长。在 CD4+ T 细胞计数低于 50 个细胞/μl 的患者中，我们发现微球菌属、不动杆菌属和克雷伯氏菌属在口腔中大量定植。在任何CD4+T细胞计数低于50个细胞/μl的患者中均未分离到草绿色链球菌。随着 CD4+ T 细胞计数提高至 51-100 个细胞/μl，草绿色链球菌菌落返回，37.5% 的患者显示出大量生长。微球菌属分离集落直至CD4+T细胞提高至300个细胞/μl。当计数 > 300 个细胞/μl 时，口腔微生物群与对照组相当。许多艾滋病毒机会性感染是由共生细菌引起的，而这些细菌对正常人来说是无害的。我们的研究的独特之处在于，此类针对艾滋病毒患者口腔共生体的研究从未有过报道。我们发现微球菌在口腔中的定殖增加。这是皮肤的正常共生体。
The study was designed to compare the oral microbiota in normal and HIV-infected individuals. The study tries to establish a significant shift in oral microflora in HIV-infected patients. Antibiotic sensitivity testing was performed to establish any rise in resistance against the antibiotics. It was a two and half year prospective study conducted in a tertiary care centre. The study group consisted of eighty subjects divided into two groups of control and HIV. The age range for this group was 9-75 years. The mean age in this group was 39.7 years. The male: female ratio was 2.75:1. Tuberculosis was the most common opportunistic infection in patients with HIV infection. The most common commensal micro organism isolated was the Viridans streptococci in 60% followed by Streptococcus pneumoniae in 23.33%. HIV Group: The most common commensal micro organism isolated was the Viridans streptococci in 42%; this was followed by the Micrococci spp. in 22% cases. S. pneumoniae was isolated in 6% of cases. The colony count for Viridans streptococci showed a heavy growth in 55.56% of cases in controls whereas the same in HIV group was 62.5%. Micrococcus spp. was isolated from 11 subjects in HIV group while it was not isolated from the controls. 50% subjects in the HIV group showed a heavy growth of Klebsiella spp. whereas controls showed only moderate and scanty growth. In patients with CD4+ T cell count less than 50 cells/mu l we found a heavy colonization of the oral cavity with Micrococcus spp., Acinetobacter and Klebsiella spp. Viridans streptococcus was not isolated in any of the patients with CD4+ T cell count less than 50 cells/mu l. As CD4+ T cells counts improved to 51-100 cells/mu l Viridans streptococcus colonies returned and 37.5% patients showed a heavy growth. Micrococcus spp. colonies were isolated till the CD4+ T cells improved up to 300 cells/mu l. At counts > 300 cells/mu l the oral microbiota became comparable to that of the controls. Many of the opportunistic infections in HIV are caused by commensal bacteria which are otherwise harmless in a normal individual. Our study is unique in that such a study of the oral commensals in HIV patients has never been reported. We found an increased colonization of the oral cavity by Micrococcus spp. which is a normal commensal of the skin.